Yin Tingyi, Feng Shuo, Zhu He, Bai Ruimin, Gan Xinyi, He Ke, Du Wenqian, Cheng Baochen, Liu Xinyi, Wang Zifeng, Zhang Hao, Zheng Yan, Liu Dingxin
Department of Dermatology, The First Affiliated Hospital, Xi'an Jiaotong University Xi'an, Shaanxi, 710061, China.
State Key Laboratory of Electrical Insulation and Power Equipment, Centre for Plasma Biomedicine, Xi'an Jiaotong University, Xi'an, Shaanxi, 710049, China.
Free Radic Biol Med. 2024 Nov 20;225:482-493. doi: 10.1016/j.freeradbiomed.2024.10.290. Epub 2024 Oct 18.
Atopic Dermatitis (AD) is a prevalent inflammatory skin disease that is currently incurable. Plasma-treated solutions (PTS) (e.g., culture media, water, or normal saline, previously exposed to plasma) are being studied as novel therapy. Recently, PTS is gaining attention due to its advantages over non-thermal plasma (also known as cold atmospheric plasma). Thus, we explore the application of PTS in treating AD. In vivo experiments demonstrated that PTS significantly alleviated AD-like symptoms. It reduced mast cell and macrophage infiltration, decreased scratching times and serum IgE levels. These therapeutic effects of PTS on AD mice were associated with the activation of the antioxidant molecule Nrf2. In vitro experiments revealed that PTS could decrease ROS level and regulate cytokine expression (such as IL-6, IL-10, IL-13 and CCL17) in TNF-α/IFN-γ-stimulated keratinocytes and LPS-stimulated M1 macrophages. Additionally, PTS could upregulate the expression of antioxidant stress molecules such as Nrf2, HO-1, NQO1 and PPAR-γ in both cell types. Overall, PTS demonstrated potent therapeutic potential for AD without notable side effects. Our research provided a promising approach to AD treatment and may serve as a potential therapeutic strategy in other inflammatory skin diseases.
特应性皮炎（AD）是一种常见的炎症性皮肤病，目前无法治愈。血浆处理溶液（PTS）（例如，先前暴露于血浆的培养基、水或生理盐水）正在作为一种新型疗法进行研究。最近，PTS因其相对于非热等离子体（也称为冷大气等离子体）的优势而受到关注。因此，我们探索了PTS在治疗AD中的应用。体内实验表明，PTS显著减轻了AD样症状。它减少了肥大细胞和巨噬细胞浸润，减少了抓挠次数和血清IgE水平。PTS对AD小鼠的这些治疗作用与抗氧化分子Nrf2的激活有关。体外实验表明，PTS可以降低TNF-α/IFN-γ刺激的角质形成细胞和LPS刺激的M1巨噬细胞中的ROS水平，并调节细胞因子表达（如IL-6、IL-10、IL-13和CCL17）。此外，PTS可以上调两种细胞类型中抗氧化应激分子如Nrf2、HO-1、NQO1和PPAR-γ的表达。总体而言，PTS对AD显示出强大的治疗潜力，且无明显副作用。我们的研究为AD治疗提供了一种有前景的方法，并可能成为其他炎症性皮肤病的潜在治疗策略。
