Topham Katherine, Stockwell Virginia, Grinstead Samuel, Mollov Dimitre
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States; USDA ARS, Horticultural Crops Disease and Pest Management Unit, Corvallis, OR, United States.
USDA ARS, Horticultural Crops Disease and Pest Management Unit, Corvallis, OR, United States.
Virus Res. 2024 Dec;350:199480. doi: 10.1016/j.virusres.2024.199480. Epub 2024 Oct 26.
New and emerging viral problems may be contributing to blueberry decline. In this research we described a new virus detected in Oregon blueberry production field and surveyed the region for its potential spread. The complete genome sequence of a putative new member of the genus Luteovirus was obtained from blueberry (Vaccinium corymbosum L.) by high throughput sequencing and 5'/3'-RACE. The new virus was tentatively named blueberry virus M (BlVM). Its genome is 5,018 nt long with four putative open reading frames. Similarly to some recently discovered luteoviruses, BlVM does not possess any movement protein (MP). Phylogenetic analysis confirmed clustering of BlVM with the group of non-MP luteoviruses, showing blueberry virus L as the most similar species. Through a small-scale high throughput sequencing survey we obtained 14 additional near complete genomic sequences. A larger survey of 2,654 samples by RT-PCR in Oregon and Washington (USA) found 52 BlVM-positive plants collected from four locations in Oregon. These findings will facilitate monitoring virus distribution and assessment of potential disease associated with this new and emerging blueberry virus.
新出现的病毒问题可能正在导致蓝莓产量下降。在本研究中，我们描述了在俄勒冈州蓝莓种植园中检测到的一种新病毒，并对该地区进行了调查，以了解其潜在传播情况。通过高通量测序和5'/3'-RACE技术，从蓝莓（Vaccinium corymbosum L.）中获得了黄症病毒属一个假定新成员的完整基因组序列。这种新病毒被暂时命名为蓝莓病毒M（BlVM）。其基因组长度为5018个核苷酸，有四个假定的开放阅读框。与一些最近发现的黄症病毒类似，BlVM不具有任何运动蛋白（MP）。系统发育分析证实，BlVM与非MP黄症病毒组聚类，显示蓝莓病毒L是最相似的物种。通过小规模高通量测序调查，我们又获得了14个近乎完整的基因组序列。在美国俄勒冈州和华盛顿州对2654个样本进行的更大规模的RT-PCR调查发现，从俄勒冈州四个地点采集的52株植物呈BlVM阳性。这些发现将有助于监测病毒分布，并评估与这种新出现的蓝莓病毒相关的潜在病害。
