Villamor D E V, Mejia A Sierra, Martin R R, Tzanetakis I E
Department of Entomology and Plant Pathology, Division of Agriculture, University of Arkansas System, Fayetteville, AR 72701.
Oregon State University and U.S. Department of Agriculture-Agricultural Research Service, Corvallis, OR 97330.
Phytopathology. 2023 Jan;113(1):98-103. doi: 10.1094/PHYTO-05-22-0186-R. Epub 2023 Jan 12.
A new blueberry virus was discovered using high-throughput sequencing. Using sequence identity values, phylogenetics, and serological and biological properties, we propose the virus, putatively named blueberry virus S (BluVS), to be a distinct species within the genus (family ). The genome was analyzed in depth, and an infectious clone was developed to initiate studies on virus pathogenicity. Agroinfiltration of the binary vector construct produced severe systemic symptoms in . Back-inoculation using sap from agroinfiltrated produced identical symptoms to the recipient plants (), and virus purification yielded flexuous carlavirus-like particles. However, unlike blueberry scorch virus (BlScV), BluVS caused symptomless infection in and reacted weakly to BlScV antibodies in an enzyme-linked immunosorbent assay. Collectively, the results provide evidence for the distinct speciation of BluVS. The availability of an infectious clone provides tools for future studies on the biology of the virus.
利用高通量测序发现了一种新的蓝莓病毒。通过序列同一性值、系统发育学以及血清学和生物学特性分析，我们提出将该病毒（暂命名为蓝莓病毒S，即BluVS）归为 属（ 科）内的一个独特物种。对其基因组进行了深入分析，并构建了感染性克隆以启动对病毒致病性的研究。二元载体构建体的农杆菌浸润在 中产生了严重的系统症状。用农杆菌浸润的 的汁液进行回接，受体植物（ ）出现了相同症状，并且病毒纯化产生了弯曲的类香石竹潜隐病毒颗粒。然而，与蓝莓焦枯病毒（BlScV）不同，BluVS在 中引起无症状感染，并且在酶联免疫吸附试验中与BlScV抗体反应较弱。总体而言，这些结果为BluVS的独特物种形成提供了证据。感染性克隆的获得为该病毒生物学的未来研究提供了工具。
