Kryzhanovskiĭ G N, Moiseev I N, Shandra A A, Makul'kin R F, Godlevskiĭ L S
Biull Eksp Biol Med. 1986 Jan;101(1):13-5.
The experiments on (CBA X C57BL/6)F1 mice have shown that regular corazol injections in subliminal doses stimulated seizure susceptibility (pharmacological kindling). Cytophotometric assay of the activity of oxidative metabolism enzymes (glutamate dehydrogenase, malate dehydrogenase, succinate dehydrogenase, alpha-oxoglutarate dehydrogenase, lactate dehydrogenase) and GABA-transaminase in the sensorimotor cortex of kindled mice in post-convulsive period, and 24 hours or 30 days after corazol injections were discontinued, has revealed some specific alterations of the enzymes under study, that suggest the existence of two phases of energy metabolism disturbances. The first phase (24 hours after corazol injections were discontinued) is characterized by intensified succinic acid oxidation, while the second phase (30 days after the last injection) is characterized by anaerobic glycolysis in neuronal and glial cells. Inhibition of GABA-transaminase activity was particularly marked in postconvulsive period. From a molecular point of view these data may be considered as enzyme disturbances during stimulation of seizure susceptability or seizure activity and as a compensation component ensuring anticonvulsive mechanisms and reparative processes (antagonistic principle of molecular mechanism regulation) during activation of antiepileptic system.
对（CBA×C57BL/6）F1小鼠进行的实验表明，以阈下剂量定期注射可拉佐会刺激癫痫易感性（药理学点燃）。在惊厥后时期以及停止注射可拉佐24小时或30天后，对点燃小鼠感觉运动皮层中氧化代谢酶（谷氨酸脱氢酶、苹果酸脱氢酶、琥珀酸脱氢酶、α-酮戊二酸脱氢酶、乳酸脱氢酶）和GABA转氨酶的活性进行细胞光度测定，发现所研究的酶存在一些特定变化，这表明能量代谢紊乱存在两个阶段。第一阶段（停止注射可拉佐24小时后）的特征是琥珀酸氧化增强，而第二阶段（最后一次注射30天后）的特征是神经元和神经胶质细胞中的无氧糖酵解。惊厥后时期GABA转氨酶活性的抑制尤为明显。从分子角度来看，这些数据可被视为癫痫易感性或癫痫活动刺激期间的酶紊乱，以及抗癫痫系统激活期间确保抗惊厥机制和修复过程的补偿成分（分子机制调节的拮抗原理）。
