Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Wollo University, Dessie, Ethiopia.
Food Science and Nutrition Research Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
J Clin Lab Anal. 2024 Nov;38(22):e25116. doi: 10.1002/jcla.25116. Epub 2024 Oct 21.
Accurate reference intervals generated from an apparently healthy population and stratified by crucial variables such as age and gender are required to guarantee appropriate interpretation of test results. Since there were no local reference intervals in the study area, the present study aimed to establish reference intervals on serum lipid profiles and electrolytes for children and adolescents in Addis Ababa.
This community-based cross-sectional study was conducted from April to October 2019. Laboratory analysis was performed using the automatic biochemical analyzer Cobas 6000 (c501) from Roche. According to Clinical and Laboratory Standards Institute (CLSI) guidelines, reference intervals for lipid profile and electrolyte tests for apparently healthy children and adolescents were established. We used a non-parametric method to calculate the 2.5th and 97.5th percentiles with a 90% confidence interval.
In children, the reference intervals for serum potassium, sodium, chloride, calcium, magnesium, and phosphate in mmol/L were 4.37-5.20, 137-145.50, 101.90-107.90, 2.34-2.70, 0.74-0.97, and 1.42-1.85, respectively; and for total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, the respective values were 100.76-171.70, 44.16-126.36, 60.60-105.60, and 31.60-53.70 in mg/dL, for both genders. For adolescents, the reference intervals were 4.03-5.58, 137-146, 98.90-120.90, 2.39-2.70, 0.73-0.96, and 0.96-1.80 for serum potassium, sodium, chloride, calcium, magnesium, and phosphate in mmol/L, respectively; and 97.20-189.10, 40.50-143.60, 41.70-120.90, and 21.30-57.0 in mg/dL for total cholesterol, triglycerides, low-density lipoprotein, and high-density lipoprotein, respectively, for both genders.
The established reference intervals in the current study revealed that both the lower and upper limits contradicted the manufacturer values as well as the available literature. The study also discovered significant gender differences in reference values for TC, TG, LDL-C, potassium, phosphate, and chloride in the adolescent age groups.
为了保证检验结果的正确解读，需要从年龄和性别等关键变量方面对来自于健康人群的准确参考区间进行分层。由于研究地区没有当地的参考区间，因此本研究旨在为亚的斯亚贝巴的儿童和青少年建立血清脂质谱和电解质的参考区间。
这是一项基于社区的横断面研究，于 2019 年 4 月至 10 月进行。使用罗氏公司的 Cobas 6000（c501）自动生化分析仪进行实验室分析。根据临床和实验室标准协会（CLSI）指南，为看似健康的儿童和青少年建立了脂质谱和电解质检测的参考区间。我们使用非参数方法计算了 90%置信区间下的 2.5%和 97.5%分位数。
在儿童中，血清钾、钠、氯、钙、镁和磷的参考区间分别为 mmol/L 4.37-5.20、137-145.50、101.90-107.90、2.34-2.70、0.74-0.97 和 1.42-1.85；总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白的参考区间分别为 mg/dL 100.76-171.70、44.16-126.36、60.60-105.60 和 31.60-53.70，男女均如此。对于青少年，血清钾、钠、氯、钙、镁和磷的参考区间分别为 mmol/L 4.03-5.58、137-146、98.90-120.90、2.39-2.70、0.73-0.96 和 0.96-1.80；总胆固醇、甘油三酯、低密度脂蛋白和高密度脂蛋白的参考区间分别为 mg/dL 97.20-189.10、40.50-143.60、41.70-120.90 和 21.30-57.0，男女均如此。
本研究建立的参考区间表明，下限和上限都与制造商的值以及现有文献相矛盾。该研究还发现，青少年组的 TC、TG、LDL-C、钾、磷和氯的参考值存在显著的性别差异。
