Groener J E, Pelton R W, Kostner G M
Clin Chem. 1986 Feb;32(2):283-6.
This simple, routine assay for measuring cholesteryl ester transfer/exchange activity in human plasma is based on the removal of interfering lipoproteins--very-low-density (VLDL) and low-density lipoproteins (LDL)--by precipitation with polyethylene glycol. High-density lipoproteins (HDL) in the samples do not affect the results. The supernate after precipitation is mixed with [14C]cholesteryl ester-labeled LDL as donor and with HDL as the acceptor for the cholesteryl ester. After incubation for 16 h at 37 degrees C, LDL is separated from HDL by precipitation with dextran sulfate and the radioactivity measured in the supernate, which contains the HDL. The assay is applicable to samples containing as much as 10 mmol of triglycerides per liter. The within-assay CV was 2.7%, the day-to-day CV 6.8%. Results compared well with those by conventional procedures.
这种用于测定人血浆中胆固醇酯转移/交换活性的简单常规检测方法，是基于用聚乙二醇沉淀去除干扰脂蛋白——极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）。样品中的高密度脂蛋白（HDL）不影响检测结果。沉淀后的上清液与作为供体的[14C]胆固醇酯标记的LDL以及作为胆固醇酯受体的HDL混合。在37℃孵育16小时后，通过用硫酸葡聚糖沉淀将LDL与HDL分离，并测量上清液（其中含有HDL）中的放射性。该检测方法适用于每升含有多达10毫摩尔甘油三酯的样品。批内变异系数为2.7%，日间变异系数为6.8%。结果与传统方法所得结果相比良好。
