Villa Sara, Aasvang Eske K, Attal Nadine, Baron Ralf, Bourinet Emmanuel, Calvo Margarita, Finnerup Nanna B, Galosi Eleonora, Hockley James R F, Karlsson Pall, Kemp Harriet, Körner Jannis, Kutafina Ekaterina, Lampert Angelika, Mürk Margarita, Nochi Zahra, Price Theodore J, Rice Andrew S C, Sommer Claudia, Taba Pille, Themistocleous Andreas C, Treede Rolf-Detlef, Truini Andrea, Üçeyler Nurcan, Bennett David L, Schmid Annina B, Denk Franziska
Wolfson Sensory, Pain and Regeneration Centre (SPaRC), King's College London, United Kingdom.
Anesthesiological Department, Center for Cancer and Organ Dysfunction, Rigshospitalet, Copenhagen University, Copenhagen, Denmark.
Pain. 2025 May 1;166(5):994-1001. doi: 10.1097/j.pain.0000000000003445. Epub 2024 Oct 16.
Neuropathic pain remains difficult to treat, with drug development hampered by an incomplete understanding of the pathogenesis of the condition, as well as a lack of biomarkers. The problem is compounded by the scarcity of relevant human peripheral tissues, including skin, nerves, and dorsal root ganglia. Efforts to obtain such samples are accelerating, increasing the need for standardisation across laboratories. In this white paper, we report on a consensus meeting attended by neuropathic pain experts, designed to accelerate protocol alignment and harmonization of studies involving relevant peripheral tissues. The meeting was held in London in March 2024 and attended by 28 networking partners, including industry and patient representatives. We achieved consensus on minimal recommended phenotyping, harmonised wet laboratory protocols, statistical design, reporting, and data sharing. Here, we also share a variety of relevant standard operating procedures as supplementary protocols. We envision that our recommendations will help unify human tissue research in the field and accelerate our understanding of how abnormal interactions between sensory neurons and their local peripheral environment contribute towards neuropathic pain.
神经性疼痛仍然难以治疗，对该病症发病机制的不完全理解以及生物标志物的缺乏阻碍了药物研发。相关人体外周组织（包括皮肤、神经和背根神经节）的稀缺使问题更加复杂。获取此类样本的工作正在加速，这增加了跨实验室标准化的需求。在本白皮书中，我们报告了一次由神经性疼痛专家参加的共识会议，旨在加速涉及相关外周组织研究的方案一致性和协调。该会议于2024年3月在伦敦举行，有28个网络合作伙伴参加，包括行业和患者代表。我们就最低推荐表型分析、统一的湿实验室方案、统计设计、报告和数据共享达成了共识。在此，我们还分享了各种相关标准操作程序作为补充方案。我们设想，我们的建议将有助于统一该领域的人体组织研究，并加速我们对感觉神经元与其局部外周环境之间的异常相互作用如何导致神经性疼痛的理解。
