Annaz Houssam, Zerrad Hamass, Moullamri Mouna, Ajaha Ayoub, Kounnoun Ayoub, Cacciola Francesco, Altemimi Ammar B, Laganà Vinci Roberto, Arakrak Abdelhay, Laglaoui Amin, Bouayad Noureddin, Rharrabe Kacem
Research team Agricultural and Aquaculture Engineering, FPL, Abdelmalek Essaadi University, Tetouan, Morocco.
Research team Biotechnologies and Biomolecular Engineering, Faculty of Science and Technology - Abdelmalek Essaadi University, Tangier, Morocco.
Chem Biodivers. 2025 Mar;22(3):e202402043. doi: 10.1002/cbdv.202402043. Epub 2024 Nov 27.
The study aims to evaluate the behavioral and insecticidal effects of Ruta angustifolia EO (RAEO) against larvae and adults of a major pest insect, Tribolium castaneum. RAEO exhibited a strong repellency against both adults and larvae where percentages as high as 74 and 72 % of PR (Percentage of Repellency) were recorded respectively, at 0.47 μL/cm after 2 h. Regarding the antifeedant bioassay, no feeding deterrence was recorded in adults while an increase in appetite was registered in larvae exhibiting -80 % of FDI recorded at 0.2 μL/pellet. In terms of feeding preference, the response significantly depended on the developmental stage (F=97.19, P<0.05) and larvae were attracted to treated pellets recording percentages of 84 and 73 % of PI (Preference index) after 5 and 30 min at 0.05 μL/pellet; on the other hand, adults expressed a repulsive behavior at 0.2 μL/pellet with a PI of -81.1 (20 min), and -69.2 % (30 min). Regarding topical application toxicity, a significant difference was observed between larvae and adults (p<0.05 F=174.56). The study promotes the use of RAEO as a repellent and bioinsecticide for the control of larvae and adults of T. castaneum.
本研究旨在评估狭叶芸香挥发油（RAEO）对主要害虫赤拟谷盗幼虫和成虫的行为及杀虫效果。RAEO对成虫和幼虫均表现出强烈的驱避作用，在2小时后，0.47微升/平方厘米的剂量下，驱避率（PR）分别高达74%和72%。关于拒食生物测定，成虫未记录到取食抑制，而在0.2微升/粒剂量下，幼虫的食欲增加，取食抑制率（FDI）为-80%。在取食偏好方面，反应显著取决于发育阶段（F = 97.19，P < 0.05），在0.05微升/粒剂量下，5分钟和30分钟后，幼虫被处理过的颗粒吸引，取食偏好指数（PI）分别为84%和73%；另一方面，成虫在0.2微升/粒剂量下表现出排斥行为，20分钟时PI为-81.1，30分钟时为-69.2%。关于局部应用毒性，幼虫和成虫之间观察到显著差异（p < 0.05，F = 174.56）。该研究促进了RAEO作为驱避剂和生物杀虫剂用于控制赤拟谷盗幼虫和成虫的应用。
