Lazarević Jelica, Kostić Igor, Šešlija Jovanović Darka, Ćalić Dušica, Milanović Slobodan, Kostić Miroslav
Institute for Biological Research "Siniša Stanković"-National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11060 Belgrade, Serbia.
Institute for Multidisciplinary Research, University of Belgrade, Kneza Višeslava 1, 11030 Belgrade, Serbia.
Plants (Basel). 2022 Dec 19;11(24):3587. doi: 10.3390/plants11243587.
The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae species. The use of essential oil compounds in pest management has been proposed as an alternative to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and a thujone-camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased at higher concentrations of the examined compounds. Camphor was more effective against larvae and the thujone-camphor mixture was more effective against adults. Additionally, adults moved faster towards the control leaf disc in the two-choice olfactometer assay if an alternative disc was treated with a thujone-camphor mixture, whereas larvae responded similarly to the two potential repellents. However, after contact with the leaf disc treated with the highest compound concentration, the larvae escaped faster from the thujone-camphor mixture than from pure camphor. In conclusion, both examined compounds are promising eco-friendly antifeedants, but their efficacy depends on the developmental stage of the beetle, compound type and applied concentration.
科罗拉多马铃薯甲虫（CPB）是经济上重要的茄科植物的一种严重害虫。有人提议使用精油化合物进行害虫管理，以此替代那些会干扰人类健康和生态系统功能的有害化学杀虫剂。我们研究了三种浓度（0.125%、0.25%和0.5%）的纯樟脑以及一种侧柏酮 - 樟脑混合物对3龄幼虫和成虫的拒食活性。根据叶片损伤程度以及CPB对处理过的叶片的回避情况来评估它们的功效。与对照相比，用马铃薯叶片进行处理显著减少了叶片损伤。在所检测化合物浓度较高时，叶片保护效果增强。樟脑对幼虫更有效，而侧柏酮 - 樟脑混合物对成虫更有效。此外，在双选嗅觉测定试验中，如果一个替代圆盘用侧柏酮 - 樟脑混合物处理，成虫会更快地移向对照叶盘，而幼虫对这两种潜在驱避剂的反应相似。然而，在接触了用最高化合物浓度处理的叶盘后，幼虫从侧柏酮 - 樟脑混合物中逃脱的速度比从纯樟脑中更快。总之，所检测的这两种化合物都是很有前景的环保型拒食剂，但它们的功效取决于甲虫的发育阶段、化合物类型和施用浓度。
