National Medical Research Center of Pediatric Hematology, Oncology and Immunology Named After Dmitry Rogachev, Russian Ministry of Healthcare, Moscow, Russian Federation.
Faculty of Physics, M. V. Lomonosov Moscow State University, Moscow, Russian Federation.
Int J Hematol. 2024 Dec;120(6):717-724. doi: 10.1007/s12185-024-03859-0. Epub 2024 Oct 22.
The recently developed platelet aggregation technique based on low-angle light scattering (LaSca) in diluted platelet-rich plasma (PRP) requires only a small sample volume and provides information about platelet aggregation and shape change. This study aimed to investigate the influence of preanalytical and analytical variables and to validate the method in a real-life pediatric hematology hospital setting.
Platelet aggregation was induced by ADP in diluted PRP in the presence of 2 mM calcium at 23 °C. The study included healthy adults (n = 30), healthy children (n = 20), and pediatric patients with suspected or diagnosed platelet function abnormalities (n = 25).
The assay parameters were stable for at least 3 h after isolation of PRP and were sensitive to plasma dilution in the range of 2-8%. The initial aggregation velocity was significantly reduced in pediatric patients compared with healthy children (p < 0.05). ADP-induced light transmission amplitude was moderately correlated with LaSca amplitude of aggregation in healthy children (p = 0.52, p < 0.05) but not in pediatric patients.
We standardized the protocol for platelet aggregation assessment by LaSca and characterized the influence of preanalytical and analytical variables on it.
最近开发的基于低角度光散射（LaSca）的血小板聚集技术在稀释的富含血小板血浆（PRP）中仅需要少量样本量，并提供关于血小板聚集和形状变化的信息。本研究旨在调查分析前和分析变量的影响，并在现实生活中的儿科血液病医院环境中验证该方法。
在 23°C 下，在 2mM 钙存在的情况下，通过 ADP 在稀释的 PRP 中诱导血小板聚集。该研究包括健康成年人（n=30）、健康儿童（n=20）和疑似或诊断为血小板功能异常的儿科患者（n=25）。
PRP 分离后至少 3 小时内，测定参数稳定，并且对 2-8%范围内的血浆稀释敏感。与健康儿童相比，儿科患者的初始聚集速度明显降低（p<0.05）。ADP 诱导的光透射幅度与健康儿童的 LaSca 聚集幅度中度相关（p=0.52，p<0.05），但与儿科患者无关。
我们通过 LaSca 对血小板聚集评估的方案进行了标准化，并描述了分析前和分析变量对其的影响。
