Wang Cong, Kong Ran, Zhong Guangcai, Li Peipei, Wang Na, Feng Ganyu, Ding Mei, Zhou Xiangxiang
Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, No.324, Jingwu Road, Jinan, 250021, Shandong, China.
Department of Hematology, Shandong Provincial Hospital, Shandong University, No.324, Jingwu Road, No.324, Jingwu Road, Jinan, 250021, Shandong, China.
Mol Biotechnol. 2024 Oct 22. doi: 10.1007/s12033-024-01264-w.
N7-methylguanosine (m7G) occurs by adding a methyl group to the N7 atom of the RNA guanine. Emerging evidence suggests that m7G modification has emerged as a crucial regulator of tumorigenesis, progression, invasion, and metastasis in multiple cancers. Nevertheless, the utility of m7G modification in diffuse large B-cell lymphoma (DLBCL) remains undefined, notably the interaction with the tumor microenvironment (TME). Here, we aimed to identify the expression profile of m7G regulators in DLBCL, construct a novel risk model, and explore their connection with TME. We initially investigated the difference and correlation in m7G regulators' expression in normal and tumor groups, classified patients by consistent clustering analysis, investigated the functional and prognostic significance of the resulting subtypes, and identified prognosis-associated genes by one-way Cox and least absolute shrinkage and selection operator (LASSO) regression calculations, and constructed a risk model. Further analysis showed that correlation among immune cell infiltration with m7G risk score and determined that it impacts the prognosis of DLBCL patients. Our research demonstrated the relevance of m7G regulators to DLBCL prognosis, providing theoretical support for precise prognostic stratification and immunotherapeutic assessment in DLBCL.
N7-甲基鸟苷（m7G）是通过在RNA鸟嘌呤的N7原子上添加一个甲基而形成的。新出现的证据表明，m7G修饰已成为多种癌症中肿瘤发生、进展、侵袭和转移的关键调节因子。然而，m7G修饰在弥漫性大B细胞淋巴瘤（DLBCL）中的作用仍不明确，尤其是与肿瘤微环境（TME）的相互作用。在此，我们旨在确定DLBCL中m7G调节因子的表达谱，构建一个新的风险模型，并探索它们与TME的联系。我们首先研究了正常组和肿瘤组中m7G调节因子表达的差异和相关性，通过一致性聚类分析对患者进行分类，研究所得亚型的功能和预后意义，并通过单因素Cox回归和最小绝对收缩和选择算子（LASSO）回归计算确定预后相关基因，进而构建风险模型。进一步分析表明，免疫细胞浸润与m7G风险评分之间存在相关性，并确定其影响DLBCL患者的预后。我们的研究证明了m7G调节因子与DLBCL预后的相关性，为DLBCL的精确预后分层和免疫治疗评估提供了理论支持。
