Zhu Fei, Sun Ke, Zhang He, Lu Jing, Guo Peng, Zhang Jiaqi, Xu Yu, Lyu Bing
School of Life Sciences Guizhou Normal University Guiyang Guizhou China.
Guizhou Academy of Forestry Guiyang Guizhou China.
Ecol Evol. 2024 Oct 22;14(10):e70480. doi: 10.1002/ece3.70480. eCollection 2024 Oct.
The interactions between hosts and the gut microbiota are intricate and can significantly affect the ecology and evolution of both parties. Various host traits, including taxonomy, diet, social behaviour, and external factors such as prey availability and the local environment, all play an important role in shaping composition and diversity of the gut microbiogta. In this study, we explored the impact of intestinal microorganisms on the host in adapting to their respective ecological niches in two species of snakes. We collected feces from and from different geographical locations and used 16S rRNA gene sequencing technology to sequence the v3-v4 region. The results revealed that there was no significant difference in the alpha diversity of intestinal microorganisms between and . The gut microbiota of all individuals comprised four main phyla: Pseudomonadota, Bacteroidota, Bacillota, and Actinomycetota. At the genus level, the genus dominated the enterobacterial microbiota in the samples from Hainan, while there was no obvious dominant genus in the enterobacterial microbiota of the samples from the other four localities. Comparative analysis of enzyme families annotated to the gut microbiota between and from the four sampling regions by CAZy carbohydrate annotation revealed that nine enzyme families differed significantly in terms of glycoside hydrolases (GHs). In addition, we compared the composition of gut microbial communities between and and investigated the impact of the differences on their functions. Our results will provide insights into the coevolution of host and gut microbes.
宿主与肠道微生物群之间的相互作用错综复杂，会显著影响双方的生态和进化。各种宿主特征，包括分类学、饮食、社会行为，以及猎物可获得性和当地环境等外部因素，在塑造肠道微生物群的组成和多样性方面都发挥着重要作用。在本研究中，我们探讨了肠道微生物对两种蛇类宿主适应各自生态位的影响。我们从不同地理位置收集了[蛇的种类1]和[蛇的种类2]的粪便，并使用16S rRNA基因测序技术对v3 - v4区域进行测序。结果显示，[蛇的种类1]和[蛇的种类2]肠道微生物的α多样性没有显著差异。所有个体的肠道微生物群包括四个主要门类：假单胞菌门、拟杆菌门、芽孢杆菌门和放线菌门。在属水平上，[具体属名]在海南样本的肠道细菌微生物群中占主导地位，而在其他四个地点样本的肠道细菌微生物群中没有明显的优势属。通过CAZy碳水化合物注释对四个采样区域的[蛇的种类1]和[蛇的种类2]肠道微生物群注释的酶家族进行比较分析发现，九个酶家族在糖苷水解酶（GHs）方面存在显著差异。此外，我们比较了[蛇的种类1]和[蛇的种类2]肠道微生物群落的组成，并研究了这些差异对其功能的影响。我们的结果将为宿主与肠道微生物的共同进化提供见解。
