培养的3T3成纤维细胞中的生长控制。V. 对具有生长抑制活性的13,000道尔顿多肽的纯化。
Growth control in cultured 3T3 fibroblasts. V. Purification of an Mr 13,000 polypeptide responsible for growth inhibitory activity.
作者信息
Hsu Y M, Wang J L
出版信息
J Cell Biol. 1986 Feb;102(2):362-9. doi: 10.1083/jcb.102.2.362.
Abstract
A growth regulatory factor, which reversibly inhibits DNA synthesis and proliferation of fibroblasts, has been isolated from medium conditioned by exposure to density-inhibited mouse 3T3 cells. This factor, termed FGR-s (13K), yielded a single polypeptide (Mr 13,000) when analyzed by SDS PAGE under both reducing and nonreducing conditions. The dose-response curve of growth inhibition by FGR-s (13K) showed that 50% inhibition of 3T3 cell proliferation was achieved at a concentration of approximately 3 ng/ml, corresponding to approximately 0.23 nM. The activity of FGR-s (13K) was depleted by passing the material over an affinity column containing the monoclonal antibody 2A4; this monoclonal antibody had been previously characterized to bind to the Mr 13,000 polypeptide. These results indicate that we have purified a growth regulatory factor that acts to inhibit the proliferation of cells in an autocrine pathway.
摘要
一种生长调节因子已从暴露于密度抑制的小鼠3T3细胞条件培养基中分离出来，该因子可可逆地抑制成纤维细胞的DNA合成和增殖。这种因子称为FGR-s（13K），在还原和非还原条件下通过SDS-PAGE分析时，产生一条单一多肽（Mr 13,000）。FGR-s（13K）生长抑制的剂量反应曲线表明，在浓度约为3 ng/ml（约0.23 nM）时可实现对3T3细胞增殖50%的抑制。通过将该物质通过含有单克隆抗体2A4的亲和柱，FGR-s（13K）的活性被耗尽；这种单克隆抗体先前已被鉴定可与Mr 13,000多肽结合。这些结果表明，我们已经纯化了一种生长调节因子，该因子通过自分泌途径抑制细胞增殖。
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