Culler M D, Tarlatzis B C, Lightman A, Fernandez L A, Decherney A H, Negro-Vilar A, Naftolin F
J Clin Endocrinol Metab. 1986 Mar;62(3):613-5. doi: 10.1210/jcem-62-3-613.
Human ovarian follicular fluid (hFF) has angiogenic activity, although the causative factors are unknown. We recently found that hFF contains renin activity which converts renin substrate to angiotensin I (AI). Since the enzymatic cleavage product of AI, angiotensin II (AII), is a potent stimulator of new vessel formation, we have examined Sephadex G-25 column fractions of hFF and extracted hFF and plasma from individual patients for AII-like immunoreactivity (AII-IR). Eluent fractions from Sephadex G-25 column chromatography of hFF had significant AII-IR which eluted in the same fractions as synthetic AII. Individual, extracted FF samples contained approximately 10 times higher levels of AII-IR than extracts of plasma from the same patients. Serial dilutions of the Sephadex column fractions and extracted FF and plasma inhibited binding of 125I-AII to rabbit anti-AII serum in a manner parallel to the inhibition caused by synthetic AII, indicating that the detected immunoreactivity was not due to non-specific assay interference. In summary, the results indicate the presence of significant AII-like immunoreactivity in hFF. AII may now be considered as a potential mediator of the angiogenic activity present in hFF and may play an important paracrine and/or autocrine role in physiologic events in the ovary.
人卵巢卵泡液（hFF）具有血管生成活性，但其致病因素尚不清楚。我们最近发现hFF含有肾素活性，可将肾素底物转化为血管紧张素I（AI）。由于AI的酶促裂解产物血管紧张素II（AII）是新血管形成的有效刺激物，我们检测了hFF的Sephadex G - 25柱层析组分，并从个体患者中提取了hFF和血浆以检测AII样免疫反应性（AII - IR）。hFF经Sephadex G - 25柱层析得到的洗脱组分具有显著的AII - IR，其洗脱组分与合成AII相同。个体提取的FF样本中AII - IR水平比同一患者血浆提取物中的高约10倍。Sephadex柱层析组分以及提取的FF和血浆的系列稀释液以与合成AII引起的抑制作用平行的方式抑制125I - AII与兔抗AII血清的结合，表明检测到的免疫反应性并非由于非特异性检测干扰。总之，结果表明hFF中存在显著的AII样免疫反应性。现在可以认为AII是hFF中存在的血管生成活性的潜在介质，并且可能在卵巢的生理事件中发挥重要的旁分泌和/或自分泌作用。
