Yan Jianing, Ye Zhichao, Wang Xiaofeng, Zhong Danyang, Wang Ziyuan, Yan Tingting, Li Tianyu, Yuan Yuyang, Liu Yu, Wang Yifan, Cai Xiujun
Department of General Surgery, Sir Run Run Shaw Hospital Affiliated to School of Medicine, Zhejiang University, Hangzhou, 310016, China.
National Engineering Research Center of Innovation and Application of Minimally Invasive Instruments, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, 310028, China.
Mater Today Bio. 2024 Oct 5;29:101290. doi: 10.1016/j.mtbio.2024.101290. eCollection 2024 Dec.
Bile duct lesion, including benign (eg. occlusion, cholelithiasis, dilatation, malformation) and malignant (cholangiocarcinoma) diseases, is a frequently encountered challenge in hepatobiliary diseases, which can be repaired by interventional or surgical procedures. A viable cure for bile duct lesions is implantation with biliary stents. Despite the placement achieved by current clinical biliary stents, the creation of functional and readily transplantable biliary stents remains a formidable obstacle. Excellent biocompatibility, stable mechanics, and absorbability are just a few benefits of using bioengineered biliary stents, which can also support and repair damaged bile ducts that drain bile. Additionally, cell sources & organoids derived from the biliary system that are loaded onto scaffolds can encourage bile duct regeneration. Therefore, the implantation of bioengineered biliary stent is considered as an ideal treatment for bile duct lesion, holding a broad potential for clinical applications in future. In this review, we look back on the development of conventional biliary stents, biodegradable biliary stents, and bioengineered biliary stents, highlighting the crucial elements of bioengineered biliary stents in promoting bile duct regeneration. After providing an overview of the various types of cell sources & organoids and fabrication methods utilized for the bioengineering process, we present the in vitro and in vivo applications of bioengineered biliary ducts, along with the latest advances in this exciting field. Finally, we also emphasize the ongoing challenges and future development of bioengineered biliary stents.
胆管病变，包括良性病变（如梗阻、胆结石、扩张、畸形）和恶性病变（胆管癌），是肝胆疾病中常见的挑战，可通过介入或手术方法进行修复。胆管病变的一种可行治疗方法是植入胆管支架。尽管目前临床胆管支架已实现放置，但制造功能性且易于移植的胆管支架仍然是一个巨大的障碍。生物工程胆管支架具有优异的生物相容性、稳定的力学性能和可吸收性等诸多优点，还能支持和修复引流胆汁的受损胆管。此外，加载到支架上的源自胆管系统的细胞来源和类器官可促进胆管再生。因此，生物工程胆管支架植入被认为是胆管病变的理想治疗方法，在未来临床应用中具有广阔潜力。在本综述中，我们回顾了传统胆管支架、可生物降解胆管支架和生物工程胆管支架的发展历程，突出了生物工程胆管支架在促进胆管再生方面的关键要素。在概述了生物工程过程中使用的各种细胞来源和类器官类型以及制造方法后，我们介绍了生物工程胆管的体外和体内应用，以及这一令人兴奋领域的最新进展。最后，我们还强调了生物工程胆管支架目前面临的挑战和未来的发展方向。
