Lee Hyun, Won Dong-Sung, Park Sinwoo, Park Yubeen, Kim Ji Won, Han Ginam, Na Yuhyun, Kang Min-Ho, Kim Seok Beom, Kang Heemin, Park Jun-Kyu, Jang Tae-Sik, Lee Sang Jin, Park Su A, Lee Sang Soo, Park Jung-Hoon, Jung Hyun-Do
Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon, 14662, Gyeonggi-do, Republic of Korea.
Department of Biotechnology, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
Bioact Mater. 2024 Mar 21;37:172-190. doi: 10.1016/j.bioactmat.2024.03.018. eCollection 2024 Jul.
Biliary strictures are characterized by the narrowing of the bile duct lumen, usually caused by surgical biliary injury, cancer, inflammation, and scarring from gallstones. Endoscopic stent placement is a well-established method for the management of biliary strictures. However, maintaining optimal mechanical properties of stents and designing surfaces that can prevent stent-induced tissue hyperplasia and biofilm formation are challenges in the fabrication of biodegradable biliary stents (BBSs) for customized treatment. This study proposes a novel approach to fabricating functionalized polymer BBSs with nanoengineered surfaces using 3D printing. The 3D printed stents, fabricated from bioactive silica poly(ε-carprolactone) (PCL) via a sol-gel method, exhibited tunable mechanical properties suitable for supporting the bile duct while ensuring biocompatibility. Furthermore, a nanoengineered surface layer was successfully created on a sirolimus (SRL)-coated functionalized PCL (fPCL) stent using Zn ion sputtering-based plasma immersion ion implantation (S-PIII) treatment to enhance the performance of the stent. The nanoengineered surface of the SRL-coated fPCL stent effectively reduced bacterial responses and remarkably inhibited fibroblast proliferation and initial burst release of SRL systems. The physicochemical properties and biological behaviors, including biocompatibility and therapeutic efficacy in the rabbit bile duct, of the Zn-SRL@fPCL stent demonstrated its potential as a versatile platform for clinical applications in bile duct tissue engineering.
胆管狭窄的特征是胆管管腔变窄，通常由外科手术胆管损伤、癌症、炎症以及胆结石瘢痕形成引起。内镜下支架置入是治疗胆管狭窄的一种成熟方法。然而，在制造用于定制治疗的可生物降解胆管支架（BBS）时，保持支架的最佳机械性能以及设计能够防止支架诱导的组织增生和生物膜形成的表面是一项挑战。本研究提出了一种使用3D打印制造具有纳米工程表面的功能化聚合物BBS的新方法。通过溶胶 - 凝胶法由生物活性二氧化硅聚（ε - 己内酯）（PCL）制造的3D打印支架，展现出适合支撑胆管同时确保生物相容性的可调机械性能。此外，使用基于锌离子溅射的等离子体浸没离子注入（S - PIII）处理，在西罗莫司（SRL）涂层的功能化PCL（fPCL）支架上成功创建了纳米工程表面层，以提高支架性能。SRL涂层fPCL支架的纳米工程表面有效降低了细菌反应，并显著抑制了成纤维细胞增殖以及SRL系统的初始突释。Zn - SRL@fPCL支架的物理化学性质和生物学行为，包括在兔胆管中的生物相容性和治疗效果，证明了其作为胆管组织工程临床应用通用平台的潜力。
