Yapar Rehime, Gündüz Özgül Soysal, Kurt Feyzan Özdal, Korkmaz Mehmet
Department of Internal Medicine, Faculty of Medicine, Pamukkale University, Denizli, Turkey.
Department of Rheumatology, Faculty of Medicine, Manisa Celal Bayar University, Manisa, Turkey.
Biol Trace Elem Res. 2024 Oct 24. doi: 10.1007/s12011-024-04425-9.
Many animal and human studies indicate that boric acid and calcium fructoborate have effects on helper T cells in immunity. The aim of our study is to evaluate the effects of boric acid and calcium fructoborate on Treg (CD4Foxp3) and Th17 (CD4Ror-γt) cell populations and related cytokine levels in mononuclear cells isolated from peripheral blood samples of rheumatoid arthritis and systemic lupus erythematosus patients. Newly diagnosed rheumatoid arthritis (n = 10) patients, systemic lupus erythematosus (n = 5) patients, and healthy individuals (n = 9) were included in this study. Consent forms were obtained from all individuals participating the study, blood samples were taken, and peripheral blood mononuclear cells were isolated. Isolated cells were exposed to low-dose and high-dose boric acid and calcium fructoborate in cell culture. Treg and Th17 cell populations were analyzed by flow cytometry after 48 h of exposure. IL-2, IL-6, IL-17, IL-23, TNF-α, and TGF-β levels in the culture medium were tested by ELISA method. At the end of the study, in healthy controls, high-dose BA improved the Treg/Th17 population but could not display similar effects on RA and SLE group. However, both boric acid and calcium fructoborate at different doses showed an increasing effect on Ror-γt in RA and SLE group. Different doses of BA and CaF treatment found to have a variable effect on cytokine. Both BA and CaF in low doses decreased TNF-α levels in RA group which shows that these boron compounds could contribute positively to the treatment of autoimmune diseases.
许多动物和人体研究表明，硼酸和果糖硼酸钙对免疫中的辅助性T细胞有影响。我们研究的目的是评估硼酸和果糖硼酸钙对从类风湿性关节炎和系统性红斑狼疮患者外周血样本中分离出的单核细胞中调节性T细胞（CD4Foxp3）和辅助性T细胞17（CD4Ror-γt）细胞群体以及相关细胞因子水平的影响。本研究纳入了新诊断的类风湿性关节炎患者（n = 10）、系统性红斑狼疮患者（n = 5）和健康个体（n = 9）。从所有参与研究的个体处获得了知情同意书，采集了血样，并分离出外周血单核细胞。将分离出的细胞在细胞培养中暴露于低剂量和高剂量的硼酸和果糖硼酸钙。暴露48小时后，通过流式细胞术分析调节性T细胞和辅助性T细胞17细胞群体。通过酶联免疫吸附测定法检测培养基中白细胞介素-2、白细胞介素-6、白细胞介素-17、白细胞介素-23、肿瘤坏死因子-α和转化生长因子-β的水平。在研究结束时，在健康对照组中，高剂量的硼酸改善了调节性T细胞/辅助性T细胞17群体，但对类风湿性关节炎和系统性红斑狼疮组未显示出类似的效果。然而，不同剂量的硼酸和果糖硼酸钙对类风湿性关节炎和系统性红斑狼疮组中的Ror-γt均显示出增加作用。发现不同剂量的硼酸和果糖硼酸钙处理对细胞因子有不同的影响。低剂量的硼酸和果糖硼酸钙均降低了类风湿性关节炎组中的肿瘤坏死因子-α水平，这表明这些硼化合物可能对自身免疫性疾病的治疗有积极作用。
