Hefei National Research Center for Physical Sciences at the Microscale, School of Chemistry and Materials Science, University of Science and Technology of China, Hefei, Anhui 230026, China.
Shanghai Synchrotron Radiation Facility, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201204, China.
J Am Chem Soc. 2024 Nov 6;146(44):30573-30583. doi: 10.1021/jacs.4c12078. Epub 2024 Oct 24.
Toehold-mediated strand displacement (TMSD) provides a versatile toolbox for developing DNA digital computing systems. Although different logic circuits with diverse functions have achieved good performance in terms of complexity and scalability, most previous DNA logic circuits perform information processing only at the molecular level, and nonspecific signal leakages are often difficult to avoid. Here, we demonstrate the feasibility of constructing leakless digital computing systems in three-dimensionally ordered colloidal supercrystals. These systems possess a unique signal leakage resistance by integrating different TMSD-based logic gates with the catalytic assembly of DNA-functionalized gold colloids. A complete set of basic Boolean logic gates and different cascaded logic circuits is constructed on the basis of the catalytic assembly strategy enabled by a facilely designed catassembler, where the output signals are recognized by determining whether specific colloidal supercrystals are formed or not. In addition, a half adder is built through a combination of XOR and AND logic gates with two distinct crystal types as readouts. Finally, a leakless two-digit DNA keypad lock for information security protection is demonstrated. The combination of TMSD-based logic circuits with the universal nanoparticle catalytic assembly offers the possibility to develop highly complicated and leakage-free digital computing systems and promotes macroscopic colloidal superlattice materials with programmable logic functions.
钉扎介导的链置换（TMSD）为开发 DNA 数字计算系统提供了一个通用的工具包。尽管具有不同功能的不同逻辑电路在复杂性和可扩展性方面都取得了良好的性能，但大多数先前的 DNA 逻辑电路仅在分子水平上进行信息处理，并且通常难以避免非特异性信号泄漏。在这里，我们展示了在三维有序胶体超晶格中构建无泄漏数字计算系统的可行性。通过将不同的基于 TMSD 的逻辑门与 DNA 功能化金胶体的催化组装相结合，这些系统具有独特的信号泄漏阻力。基于易于设计的 catassembler 实现的催化组装策略，构建了一套完整的基本布尔逻辑门和不同的级联逻辑电路，其中输出信号通过确定是否形成特定的胶体超晶格来识别。此外，通过将 XOR 和 AND 逻辑门与两种不同的晶体类型组合作为读出，构建了一个无泄漏的半加器。最后，展示了用于信息安全保护的无泄漏两位数 DNA 键盘锁。基于 TMSD 的逻辑电路与通用纳米颗粒催化组装的结合为开发高度复杂和无泄漏的数字计算系统提供了可能性，并促进了具有可编程逻辑功能的宏观胶体超晶格材料的发展。
