Xu Hu, Jia Dongxu, Guo Shuaihang, Zheng Xinyan, Yang Wei, Chen Hong, Zhang Yanxia, Yu Qian
State and Local Joint Engineering Laboratory for Novel Functional Polymeric Materials, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.
Department of Cardiovascular Surgery of the First Affiliated Hospital and Institute for Cardiovascular Science, Suzhou Medical College of Soochow University, Soochow University, Suzhou 215007, PR China.
J Colloid Interface Sci. 2025 Feb;679(Pt B):191-200. doi: 10.1016/j.jcis.2024.10.109. Epub 2024 Oct 20.
Biofilms formed by pathogenic bacteria on biomedical devices and implants pose a considerable challenge due to their resistance to conventional treatments and their role in severe infections. Preventing biofilm formation is strategically more advantageous than attempting to eliminate the mature biofilms, particularly in addressing the persistence of such formations. In this context, a dual-action antibiofilm coating is developed, utilizing S-nitrosothiols functionalized candle soot (CS), which capitalizes on CS's strong light absorption for photothermal therapy and the controlled release of nitric oxide (NO) from S-nitrosothiols to inhibit biofilm formation. This coating exhibits stable and efficient light-to-heat conversion, along with the ability to release NO gradually at physiological temperatures and to rapidly release NO on demand when triggered by a near-infrared (NIR) laser. Under NIR irradition, the coating generates heat swiftly and releases substantial amounts of NO, which synergistically disrupts bacterial membranes, leading to the leakage of intracellular components and the effective eradication of surface-adhered bacteria. In the absence of NIR irradiation, the coating continuously releases low concentrations of NO, which depletes exopolysaccharides and impedes biofilm formation. The antibiofilm efficacy of this coating is assessed against Staphylococcus aureus and Pseudomonas aeruginosa, demonstrating marked reductions in bacterial viability and biofilm formation in vitro. Additionally, the coating exhibits minimal cytotoxicity and can be easily applied to diverse substrates. This study underscores the potential of this coating as a broad-spectrum, non-toxic approach for preventing biofilm-related complications in biomedical applications.
致病细菌在生物医学设备和植入物上形成的生物膜对传统治疗具有抗性，且在严重感染中起作用，因此构成了相当大的挑战。从战略角度来看，防止生物膜形成比试图消除成熟生物膜更具优势，特别是在解决此类生物膜的持续性问题方面。在此背景下，开发了一种双功能抗生物膜涂层，该涂层利用S-亚硝基硫醇功能化的蜡烛烟灰（CS），它利用CS的强光吸收能力进行光热疗法，并通过S-亚硝基硫醇控制释放一氧化氮（NO）来抑制生物膜形成。这种涂层表现出稳定且高效的光热转换能力，以及在生理温度下逐渐释放NO和在近红外（NIR）激光触发时按需快速释放NO的能力。在近红外辐射下，涂层迅速产热并释放大量NO，二者协同破坏细菌膜，导致细胞内成分泄漏并有效根除表面附着的细菌。在没有近红外辐射的情况下，涂层持续释放低浓度的NO，消耗胞外多糖并阻碍生物膜形成。针对金黄色葡萄球菌和铜绿假单胞菌评估了该涂层的抗生物膜功效，结果表明其在体外显著降低了细菌活力和生物膜形成。此外，该涂层的细胞毒性极小，并且可以轻松应用于各种基材。这项研究强调了这种涂层作为一种广谱、无毒方法在生物医学应用中预防生物膜相关并发症的潜力。
