Li Yu-Lin, Chen Bo-Yan, Feng Ze-Hao, Zhou Lu-Jun, Liu Ting, Lin Wen-Zhen, Zhu Hong, Xu Shuo, Bai Xue-Bing, Meng Xiao-Qian, Zhang Jun, Liu Yan, Pu Jun, Jiang Meng, Duan Sheng-Zhong
Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China; Department of General Dentistry, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.
Laboratory of Oral Microbiota and Systemic Diseases, Shanghai Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.
J Adv Res. 2024 Oct 22. doi: 10.1016/j.jare.2024.10.009.
The significance of oral/gut microbiota in acute myocardial infarction (AMI) has been increasingly appreciated. However, correlations between oral/gut microbiota and AMI parameter, as well as the key microbiota that may have a crucial function in this process, remain unclear.
To investigate the composition and structure of oral and gut microbiota associated with AMI and explore the roles of specific bacterial species in the progression of AMI.
We conducted a case-control study with 37 AMI patients and 36 controls. Oral and gut sample were collected and sequenced. Using correlation analysis, we combined bioinformatics data with AMI clinical parameters and obtained heatmaps of correlation coefficients. Additionally, we used antibiotics to eliminate the gut microbiota of C57BL/6J mice, followed by the transplantation of selected bacteria to verify the gut colonization of oral bacteria and their impact on AMI.
The component of oral and gut microbiota of AMI group showed significant alterations when compared to the control group. 17 salivary genera, 21 subgingival genera, and 8 gut genera in AMI group substantially differed from those in control group. Additionally, 19 genera from saliva, 19 genera from subgingival plaque, and 11 genera from feces substantially correlated with AMI clinical parameters. Orally administrated S.o (Streptococcus oralis subsp. dentisani), S.p (Streptococcus parasanguinis), and S.s (Streptococcus salivarius) were able to colonize in the gut and exacerbate myocardial infarction.
There is a strong correlation between oral/gut microbiota and AMI. Streptococcus spp. is capable to transmit from oral to gut and exacerbate myocardial infarction in mice. Monitoring and control of specific oral microbiota may be an effective new strategy for improving the therapy of AMI.
口腔/肠道微生物群在急性心肌梗死（AMI）中的重要性已日益受到重视。然而，口腔/肠道微生物群与AMI参数之间的相关性，以及在此过程中可能起关键作用的关键微生物群仍不清楚。
研究与AMI相关的口腔和肠道微生物群的组成和结构，并探讨特定细菌种类在AMI进展中的作用。
我们进行了一项病例对照研究，纳入37例AMI患者和36例对照。收集口腔和肠道样本并进行测序。通过相关性分析，我们将生物信息学数据与AMI临床参数相结合，获得相关系数热图。此外，我们使用抗生素清除C57BL/6J小鼠的肠道微生物群，然后移植选定的细菌，以验证口腔细菌在肠道中的定植及其对AMI的影响。
与对照组相比，AMI组口腔和肠道微生物群的组成有显著改变。AMI组的17个唾液菌属、21个龈下菌属和8个肠道菌属与对照组有很大差异。此外，来自唾液的19个菌属、来自龈下菌斑的19个菌属和来自粪便的11个菌属与AMI临床参数有显著相关性。口服口腔链球菌亚种牙本质亚种（S.o）、副血链球菌（S.p）和唾液链球菌（S.s）能够在肠道定植并加重心肌梗死。
口腔/肠道微生物群与AMI之间存在密切相关性。链球菌属能够从口腔传播到肠道并加重小鼠的心肌梗死。监测和控制特定的口腔微生物群可能是改善AMI治疗的一种有效的新策略。
