Ayodele Tawakalt, Liadi Musiliu, Tijani Abodunrin Tirmidhi, Alarape Kudirat, Bitrus Christiana, Clementson Clairmont L, Hammed Ademola
Environmental and Conservation Sciences, North Dakota State University, Fargo, ND 58102, USA.
Agricultural and Biosystems Engineering, North Dakota State University, Fargo, ND 58108, USA.
BioTech (Basel). 2024 Oct 19;13(4):43. doi: 10.3390/biotech13040043.
The production of microbial proteins (MPs) has emerged as a critical focus in biotechnology, driven by the need for sustainable and scalable alternatives to traditional protein sources. This study investigates the efficacy of two experimental setups in producing MPs using the nitrogen-fixing bacterium . , known for its facultative anaerobic growth and capability to fix atmospheric nitrogen, offers a promising avenue for environmentally friendly protein production. This research compares the performance of a simple bubble column (BC) bioreactor, which promotes efficient mixing and cross-membrane gas transfer, with static fermentation, a traditional method lacking agitation and aeration. The study involved the parallel cultivation of in both systems, with key parameters such as microbial growth, glucose utilization, protein concentration, and metabolite profiles monitored over a 48 h period. The results indicate that the BC bioreactor consistently outperformed static fermentation regarding the growth rate, protein yield, and glucose utilization efficiency. The BC exhibited a significant increase in protein production, reaching 299.90 µg/mL at 48 h, compared to 219.44 µg/mL in static fermentation. The organic acid profile reveals both synthesis and utilization regimes of varying patterns. These findings highlight the advantages of the BC bioreactor for MP production, particularly its ability to maintain aerobic conditions that support higher growth and yield.
由于需要可持续且可扩展的传统蛋白质来源替代品，微生物蛋白（MPs）的生产已成为生物技术的一个关键重点。本研究调查了两种实验装置在利用固氮细菌生产MPs方面的效果。以其兼性厌氧生长和固定大气氮的能力而闻名，为环境友好型蛋白质生产提供了一条有前景的途径。本研究比较了促进高效混合和跨膜气体转移的简单鼓泡塔（BC）生物反应器与缺乏搅拌和曝气的传统静态发酵方法的性能。该研究涉及在两个系统中平行培养，在48小时内监测微生物生长、葡萄糖利用、蛋白质浓度和代谢物谱等关键参数。结果表明，在生长速率、蛋白质产量和葡萄糖利用效率方面，BC生物反应器始终优于静态发酵。BC显示蛋白质产量显著增加，在48小时时达到299.90μg/mL，而静态发酵为219.44μg/mL。有机酸谱揭示了不同模式的合成和利用机制。这些发现突出了BC生物反应器在MP生产方面的优势，特别是其维持支持更高生长和产量的有氧条件的能力。
