Sobhanifar Fatemeh, Tavakoli Fatemeh, Eslami Hosein, Dalir Abdolahinia Elahe, Pakdel Farzaneh, Motahari Paria, Seyyedshariatdoust Sana, Barzegar Farshad, Valizadeh Nasrin, Jafari Bahare
Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Oral and Maxillofacial Medicine Department, School of Dentistry, Shiraz University of Medical Sciences, Shiraz, Iran.
J Lasers Med Sci. 2024 Sep 24;15:e46. doi: 10.34172/jlms.2024.46. eCollection 2024.
Laser therapy employs a concentrated and slender light beam to eliminate or eradicate cancerous cells and pre-cancerous abnormalities. The specific wavelength of 808 nm light is preferentially absorbed by tumor cells compared to healthy cells. This study aimed to assess the combined therapeutic impact of laser and gold, given that gold exhibits photothermal properties when exposed to laser radiation. In this in vitro study, two cell lines, namely healthy HuGu cells (human gingival fibroblast cells) and head and neck cancer cells (HN-5), were obtained from the Pasteur Institute. The effect of the laser diode with a density of 3 J/cm and wavelength of 808 nm on the proliferation and the survival rate of oral squamous cell carcinoma (HN-5) and human gingival fibroblast (HUGU) was assessed in 60seconds. MTT assay, DAPI test, and trypan blue staining were used to determine the growth and proliferation of HN-5 and HUGU cell lines. Findings showed that the laser diode along with gold decreased the rate of proliferation and survival cells in HN-5 compared to healthy cells. The changes in the cell population treated with gold and laser diode 808 were insignificant. Findings reveal that using a low-power laser can effectively inhibit the growth of oral cancer cells. It seems that photothermal therapy is a novel approach to oral cancer therapy.
激光疗法采用集中且细长的光束来消除或根除癌细胞及癌前异常病变。与健康细胞相比，808纳米波长的特定光更易被肿瘤细胞吸收。鉴于金在激光辐射下具有光热特性，本研究旨在评估激光与金的联合治疗效果。在这项体外研究中，从巴斯德研究所获取了两种细胞系，即健康的胡古细胞（人牙龈成纤维细胞）和头颈癌细胞（HN - 5）。评估了密度为3 J/cm且波长为808纳米的激光二极管在60秒内对口腔鳞状细胞癌（HN - 5）和人牙龈成纤维细胞（HUGU）增殖及存活率的影响。采用MTT法、DAPI检测和台盼蓝染色来确定HN - 5和HUGU细胞系的生长和增殖情况。研究结果表明，与健康细胞相比，激光二极管与金联合使用降低了HN - 5细胞的增殖率和存活细胞数量。用金和808纳米激光二极管处理的细胞群体变化不显著。研究结果显示，使用低功率激光可有效抑制口腔癌细胞的生长。光热疗法似乎是一种治疗口腔癌的新方法。
