Anishya Daphane, Jain Ravindra K
Department of Orthodontics, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India.
J Orthod Sci. 2024 Sep 17;13:31. doi: 10.4103/jos.jos_29_24. eCollection 2024.
Surface roughness of arch wires directly impacts their corrosion behavior, friction resistance, and plaque accumulation, which may hinder tooth movement and lead to dental caries.
The study aims to synthesize vanillin-mediated silver nanoparticles (AgNPs), characterize them, assess surface roughness and cytotoxicity of arch wires after silver nanoparticle coating, and test their antibacterial properties.
Nine copper-nickel-titanium arch wires (CuNiTi) were cut into equal pieces. Three were sent for surface roughness assessment, three for cytotoxicity, and three for antibacterial testing. Dip coating of wires was done using the sol-gel thin film method. The surface roughness (Ra) before and after coating was evaluated using scanning electron microscopy and atomic force microscopy. Cytotoxicity testing was done with a (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay using gingival fibroblasts. Statistical analysis was done using SPSS software. Antibacterial activity against was tested using the Agar-well diffusion method.
The CuNiTi wires were coated successfully, and the coating appeared homogeneous. The mean Ra after coating (297.3+/- 30.4 nm) was significantly less than that before coating (339.7+/-49.2 nm). AgNPs showed minimal cytotoxicity against human gingival fibroblasts at different concentrations. Optical microscopy showed over 90% viability between 12.5 and 100 µg/ml. At 100 µg/ml, only 80% of cells remained viable. AgNP coating is biocompatible at concentrations up to 75 µg/ml. There was a significant intergroup difference in the zone of inhibition (antibacterial activity) noted with higher values in noncoated wires. ( value <0.007).
AgNPs coated on CuNiTi arch wires showed reduced surface roughness and minimal cytotoxic effects on human gingival cells and good antibacterial activity against S. mutans compared to noncoated arch wires.
弓丝的表面粗糙度直接影响其腐蚀行为、摩擦阻力和菌斑堆积，这可能会阻碍牙齿移动并导致龋齿。
本研究旨在合成香草醛介导的银纳米颗粒（AgNPs），对其进行表征，评估银纳米颗粒涂层后弓丝的表面粗糙度和细胞毒性，并测试其抗菌性能。
将九根铜镍钛弓丝（CuNiTi）切成相等的片段。三根送去进行表面粗糙度评估，三根进行细胞毒性测试，三根进行抗菌测试。采用溶胶-凝胶薄膜法对弓丝进行浸涂。使用扫描电子显微镜和原子力显微镜评估涂层前后的表面粗糙度（Ra）。使用牙龈成纤维细胞通过（3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐）试验进行细胞毒性测试。使用SPSS软件进行统计分析。使用琼脂孔扩散法测试对变形链球菌的抗菌活性。
CuNiTi弓丝成功涂层，涂层呈现均匀。涂层后的平均Ra（297.3±30.4nm）显著低于涂层前（339.7±49.2nm）。AgNPs在不同浓度下对人牙龈成纤维细胞显示出最小的细胞毒性。光学显微镜显示在12.5至100μg/ml之间存活率超过90%。在100μg/ml时，仅80%的细胞仍具有活力。AgNP涂层在浓度高达75μg/ml时具有生物相容性。在抑菌圈（抗菌活性）方面观察到显著的组间差异，未涂层的弓丝值更高（值<0.007）。
与未涂层的弓丝相比，涂覆在CuNiTi弓丝上的AgNPs显示出降低的表面粗糙度，对人牙龈细胞的细胞毒性最小，并且对变形链球菌具有良好的抗菌活性。
