Quispe-Siccha Rosa M, Medina-Sandoval Osvaldo I, Estrada-Tinoco Abraham, Pedroza-Pérez Jorge A, Martínez-Tovar Adolfo, Olarte-Carrillo Irma, Cerón-Maldonado Rafael, Reding-Bernal Arturo, López-Alvarenga Juan C
Research and Technological Development Unit, Research Department, General Hospital of Mexico, "Dr. Eduardo Liceaga", Mexico City 06726, Mexico.
Interdisciplinary Biotechnology Professional Unit, National Polytechnic Institute, Mexico City 07340, Mexico.
Gels. 2024 Oct 19;10(10):668. doi: 10.3390/gels10100668.
Polyvinyl alcohol (PVA) hydrogels have a wide range of applications in the pharmaceutical and biomedicine fields due to their exceptional biophysical properties. The study focuses on preparing and characterizing capsule-shaped PVA hydrogels to enhance their biocompatibility and porosity for controlled glucose release and cell proliferation. The hydrogels were prepared using different concentrations (Cs) and molecular weights (MWs) of PVA, with two different lengths, A (10 mm) and B (20 mm), to control glucose release over 60 min. The preparation process involved PVA gel preparation and PVA hydrogel formation. A total of 500 µL of glucose was injected into all dehydrated hydrogels in groups A and B. Glucose release was studied by immersing the hydrogels in saline at 37 °C with stirring at 500 rpm. The SUP-B15 cell line was grown in six A1 hydrogels for biocompatibility testing. The results indicate that all hydrogels remained stable at 37 °C without degrading. Those with a higher C and MW exhibited a denser and less porous structure, lower glucose storage capacity, and higher elongation at break. Significant differences in glucose release, diffusion speed, and flux were observed, which were more evident in A1 > A4, B1 > B4, and B1 > A1 over 60 min. A1 and B1 had higher values because their higher porosity distribution allowed glucose to diffuse more easily. B1, being larger, has more glucose due to its increased length. The cell growth response and viability at 48 h in contact with the hydrogels was similar to that of the control (4.5 × 10 cells/mL, 98.5% vs. 4.8 × 10 cells/mL, 99.7% viability), thus demonstrating biocompatibility. The hydrogels effectively released glucose over 60 min, with variations based on porosity, C, MW, and length, and demonstrated good biocompatibility with the cell line.
聚乙烯醇（PVA）水凝胶因其优异的生物物理特性在制药和生物医学领域有着广泛的应用。该研究聚焦于制备并表征胶囊状PVA水凝胶，以提高其生物相容性和孔隙率，实现葡萄糖的可控释放以及细胞增殖。使用不同浓度（Cs）和分子量（MWs）的PVA制备水凝胶，其长度有两种，分别为A（10毫米）和B（20毫米），用于在60分钟内控制葡萄糖释放。制备过程包括PVA凝胶制备和PVA水凝胶形成。向A组和B组所有脱水水凝胶中均注入500微升葡萄糖。通过将水凝胶置于37℃的盐水中并以500转/分钟搅拌来研究葡萄糖释放情况。将SUP - B15细胞系培养于六个A1水凝胶中进行生物相容性测试。结果表明，所有水凝胶在37℃下保持稳定，未发生降解。具有较高C和MW的水凝胶呈现出更致密、孔隙率更低的结构，葡萄糖储存能力较低，且断裂伸长率较高。观察到葡萄糖释放、扩散速度和通量存在显著差异，在60分钟内，A1 > A4、B1 > B4以及B1 > A1的差异更为明显。A1和B1具有较高的值，因为其较高的孔隙率分布使葡萄糖更容易扩散。B1较大，因其长度增加而含有更多葡萄糖。与水凝胶接触48小时时细胞的生长反应和活力与对照组相似（对照组细胞密度为4.5×10个细胞/毫升，活力为98.5%；实验组细胞密度为4.8×10个细胞/毫升，活力为99.7%），从而证明了生物相容性。水凝胶在60分钟内有效释放葡萄糖，释放情况因孔隙率、C、MW和长度而异，并且与细胞系表现出良好的生物相容性。
