Zhao Shuai, Qing Jun, Yang Zhiguo, Tian Tian, Yan Yanqiu, Li Hui, Bai Yu'e
College of Forestry, Inner Mongolia Agricultural University, Hohhot 010019, China.
Institute of Desertification Studies, Inner Mongolia Academy of Forestry, Hohhot 010019, China.
Curr Issues Mol Biol. 2024 Oct 14;46(10):11375-11393. doi: 10.3390/cimb46100678.
is an ancient remnant species from the Mediterranean displaying characteristics such as high-temperature tolerance, drought resistance, cold resistance, and adaptability to impoverished soil. In the case of high-temperature tolerance, heat shock transcription factors (HSFs) are integral transcriptional regulatory proteins exerting a critical role in cellular processes. Despite extensive research on the HSF family across various species, there has been no analysis specifically focused on . In this study, we identified 24 members of the gene family based on the genome database of , which were unevenly distributed over 9 chromosomes. Phylogenetic analysis showed that these 24 members can be categorized into 5 primary classes consisting of a total of 13 subgroups. Analysis of the physical and chemical properties revealed significant diversity among these proteins. With the exception of the AmHSFB3 protein, which is localized in the cytoplasm, all other AmHSF proteins were found to be situated in the nucleus. Comparison of amino acid sequences revealed that all AmHSF proteins contain a conserved DNA-binding domains structure, and the DNA-binding domains and oligomerization domains of the gene exhibit conservation with counterparts across diverse species; we investigated the collinearity of genes in relation to those of three other representative species. Through GO enrichment analysis, evidence emerged that genes are involved in heat stress responses and may be involved in multiple transcriptional regulatory pathways that coordinate plant growth and stress responses. Finally, through a comprehensive analysis using transcriptome data, we examined the expression levels of 24 under 45 °C. The results revealed significant differences in the expression profiles of at different time intervals during exposure to high temperatures, highlighting their crucial role in responding to heat stress. In summary, these results provide a better understanding of the role and regulatory mechanisms of HSF in the heat stress response of , meanwhile also establishing a foundation for further exploration of the biological functions of in the adversity response of
是一种来自地中海的古老残遗物种，具有耐高温、抗旱、抗寒以及对贫瘠土壤的适应性等特征。就耐高温而言，热激转录因子（HSFs）是重要的转录调节蛋白，在细胞过程中发挥关键作用。尽管对不同物种的HSF家族进行了广泛研究，但尚未有专门针对[具体物种]的分析。在本研究中，我们基于[具体物种]的基因组数据库鉴定出该基因家族的24个成员，它们不均匀地分布在9条染色体上。系统发育分析表明，这24个成员可分为5个主要类别，共13个亚组。对其理化性质的分析揭示了这些蛋白质之间存在显著差异。除了定位于细胞质的AmHSFB3蛋白外，所有其他AmHSF蛋白都位于细胞核中。氨基酸序列比较表明，所有AmHSF蛋白都含有保守的DNA结合结构域，并且该基因的DNA结合结构域和寡聚化结构域与不同物种的对应结构域具有保守性；我们研究了该基因与其他三个代表性物种基因的共线性。通过GO富集分析，有证据表明该基因参与热应激反应，可能参与协调植物生长和应激反应的多个转录调控途径。最后，通过使用转录组数据的综合分析，我们检测了45℃下24个[具体基因]的表达水平。结果显示，在高温暴露的不同时间间隔内，[具体基因]的表达谱存在显著差异，突出了它们在应对热应激中的关键作用。总之，这些结果有助于更好地理解HSF在[具体物种]热应激反应中的作用和调控机制，同时也为进一步探索[具体物种]在逆境反应中的生物学功能奠定了基础。
