Gao Fei, Wang Jianyue, Wei Shanjun, Li Zhanglei, Wang Ning, Li Huayun, Feng Jinchao, Li Hongjie, Zhou Yijun, Zhang Feixiong
College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China.
College of Life Science, Capital Normal University, Beijing 100048, China.
PLoS One. 2015 Apr 29;10(4):e0124382. doi: 10.1371/journal.pone.0124382. eCollection 2015.
Ammopiptanthus mongolicus (Maxim. Ex Kom.) Cheng f., a relic tree of the Tertiary period, plays a critical role in maintaining desert ecosystems in the Mid-Asia region. Genome-scale gene expression profiling studies will provide deep insight into the molecular mechanism underlying the drought tolerance of A. mongolicus. In the present study, we investigated the transcriptional changes induced by drought treatment in A. mongolicus leaves by establishing a comprehensive transcriptome database and then performing a Digital Gene Expression (DGE) analysis using Solexa sequencing technology. A comprehensive transcriptome database was obtained by assembling the Illumina unigenes with expressed sequence tags (EST) available publicly, and other high throughput sequencing data. To analyze the dynamic and complicated gene regulation network during PEG6000-induced drought treatment in leaves of A. mongolicus, a time-course gene expression analysis was performed using tag-based DGE technology, which identified 437, 1,247 and 802 differentially expressed transcripts in 1, 24 and 72 h drought stress libraries, respectively. GO and KEGG analyses revealed hormone signal transduction and phenylpropanoid biosynthesis were enriched during drought treatment. A batch of drought-regulated transcription factor transcripts were identified, including the subsets of HD-ZIP, bZIP, WRKY, AP2/ERF and bHLH family members, which may play roles in drought response in A. mongolicus. The sequence collection assembled in the present study represents one of the most comprehensive transcriptome databases for A. mongolicus currently. The differentially expressed transcripts identified in our study provide a good start for identifying the key genes in stress response and performing functional analysis to reveal their roles in stress adaptation in planta.
蒙古沙冬青（Ammopiptanthus mongolicus (Maxim. Ex Kom.) Cheng f.）是第三纪的孑遗树种，在维持中亚地区沙漠生态系统方面发挥着关键作用。全基因组规模的基因表达谱研究将为深入了解蒙古沙冬青耐旱性的分子机制提供见解。在本研究中，我们通过建立一个全面的转录组数据库，然后使用Solexa测序技术进行数字基因表达（DGE）分析，研究了干旱处理诱导的蒙古沙冬青叶片转录变化。通过将Illumina单基因与公开可用的表达序列标签（EST）以及其他高通量测序数据进行组装，获得了一个全面的转录组数据库。为了分析聚乙二醇6000诱导的蒙古沙冬青叶片干旱处理过程中动态复杂的基因调控网络，使用基于标签的DGE技术进行了时间进程基因表达分析，该分析分别在干旱胁迫1、24和72小时的文库中鉴定出437、1247和802个差异表达转录本。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，干旱处理期间激素信号转导和苯丙烷生物合成途径显著富集。鉴定出一批受干旱调控的转录因子转录本，包括HD-ZIP、bZIP、WRKY、AP2/ERF和bHLH家族成员的子集，它们可能在蒙古沙冬青的干旱响应中发挥作用。本研究中组装的序列集合代表了目前蒙古沙冬青最全面的转录组数据库之一。我们研究中鉴定出的差异表达转录本为鉴定胁迫响应中的关键基因以及进行功能分析以揭示它们在植物胁迫适应中的作用提供了一个良好的开端。
