Navarro-Hernandez Itze C, Reyes-Huerta Raúl F, Cañez-Hernández Mariana, Torres-Ruiz Jiram, Carrillo-Vázquez Daniel A, Whittall-García Laura P, Meza-Sánchez David E, Juárez-Vega Guillermo, Gómez-Martin Diana, Hernández-Hernández José M, Maravillas-Montero José L
B Cell Immunology Laboratory, Coordinación de la Investigación Científica, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
Departmento de Biología Celular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City 07360, Mexico.
Diagnostics (Basel). 2024 Oct 12;14(20):2271. doi: 10.3390/diagnostics14202271.
Systemic lupus erythematosus (SLE) is an autoimmune disorder that often leads to kidney injury, known as lupus nephritis (LN). Although renal biopsy is the primary way to diagnose LN, it is invasive and not practical for regular monitoring. As an alternative, several groups have proposed urinary extracellular vesicles (uEVs) as potential biomarkers for LN, as recent studies have shown their significance in reflecting kidney-related diseases. As a result, we developed a flow cytometry approach that allowed us to determine that LN patients exhibited a significantly higher total uEV concentration compared to SLE patients without kidney involvement. Additionally, an analysis of different-sized uEV subsets revealed that microvesicles ranging from 0.3 to 0.5 μm showed the most promise for distinguishing LN. These findings indicate that evaluating uEV concentration and size distribution could be a valuable diagnostic and monitoring tool for LN, pending further validation in more comprehensive studies.
系统性红斑狼疮（SLE）是一种自身免疫性疾病，常导致肾脏损伤，即狼疮性肾炎（LN）。虽然肾活检是诊断LN的主要方法，但它具有侵入性，且不适用于常规监测。作为一种替代方法，一些研究小组提出将尿液细胞外囊泡（uEVs）作为LN的潜在生物标志物，因为最近的研究表明它们在反映肾脏相关疾病方面具有重要意义。因此，我们开发了一种流式细胞术方法，该方法使我们能够确定，与无肾脏受累的SLE患者相比，LN患者的总uEV浓度显著更高。此外，对不同大小的uEV亚群的分析表明，直径在0.3至0.5μm之间的微泡在区分LN方面最具潜力。这些发现表明，评估uEV浓度和大小分布可能是一种有价值的LN诊断和监测工具，有待在更全面的研究中进一步验证。
