Bidzhieva Salimat K, Tourova Tatyana P, Kadnikov Vitaly V, Samigullina Salima R, Sokolova Diyana S, Poltaraus Andrey B, Avtukh Alexander N, Tereshina Vera M, Beletsky Alexey V, Mardanov Andrey V, Nazina Tamara N
Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia.
Institute of Bioengineering, Research Center of Biotechnology, Russian Academy of Sciences, Moscow 119071, Russia.
Biology (Basel). 2024 Oct 7;13(10):800. doi: 10.3390/biology13100800.
The search for the microorganisms responsible for sulfide formation and corrosion of steel equipment in the oil fields of Tatarstan (Russia) resulted in the isolation of a new halotolerant strictly anaerobic sulfate-reducing bacterium, strain 5S69. The cells were motile curved Gram-negative rods. Optimal growth was observed in the presence of 2.0-4.0% (/) NaCl, at pH 6.5, and at 23-28 °C under sulfate-reducing conditions. The isolate was capable of chemoorganotrophic growth with sulfate and other sulfoxides as electron acceptors, resulting in sulfide formation; and of pyruvate fermentation resulting in formation of H and acetate. The strain utilized lactate, pyruvate, ethanol, methanol, fumarate, and fructose, as well as H/CO/acetate for sulfate reduction. The genome size of the type strain 5S69 was 4.16 Mb with a G + C content of 63.0 mol%. On the basis of unique physiological properties and results of the 16S rRNA gene-based phylogenetic analysis, phylogenomic analysis of the 120 conserved single copy proteins and genomic indexes (ANI, AAI, and dDDH), assigning the type strain 5S69 ((VKM B-3653 = KCTC 25499) to a new species within the genus , is suggested, with the proposed name sp. nov. Genome analysis of the new isolate showed several genes involved in sulfate reduction and its sulfide-producing potential in oil fields with high saline formation water.
对俄罗斯鞑靼斯坦油田中导致硫化物形成和钢铁设备腐蚀的微生物进行研究，结果分离出一种新的耐盐严格厌氧硫酸盐还原菌，菌株5S69。细胞为具运动性的弯曲革兰氏阴性杆菌。在2.0 - 4.0%（/）NaCl存在下、pH 6.5以及23 - 28°C的硫酸盐还原条件下观察到最佳生长。该分离株能够以硫酸盐和其他亚砜作为电子受体进行化学有机营养生长，从而形成硫化物；还能进行丙酮酸发酵形成氢气和乙酸盐。该菌株利用乳酸盐、丙酮酸盐、乙醇、甲醇、富马酸盐和果糖，以及氢气/二氧化碳/乙酸盐进行硫酸盐还原。模式菌株5S69的基因组大小为4.16 Mb，G + C含量为63.0 mol%。基于独特的生理特性以及基于16S rRNA基因的系统发育分析结果、对120个保守单拷贝蛋白的系统基因组分析和基因组指数（ANI、AAI和dDDH），建议将模式菌株5S69（（VKM B - 3653 = KCTC 25499）归入属内一个新物种，提议名称为 sp. nov.。对新分离株的基因组分析显示了几个参与硫酸盐还原及其在高盐水层油田中产生硫化物潜力的基因。
