Graduate School of Environmental Science, Hokkaido University, Kita-10, Nishi-5, Kita-ku, Sapporo, 060-0810, Japan.
The Institute of Low Temperature Science, Hokkaido University, Kita-19, Nishi-8, Kita-ku, Sapporo, 060-0819, Japan.
Arch Microbiol. 2022 May 9;204(6):307. doi: 10.1007/s00203-022-02870-5.
A novel mesophilic and neutrophilic sulfate-reducing bacterium, strain SF6, was isolated from sediment of a brackish lake in Japan. Cells of strain SF6 were motile and rod-shaped with length of 1.2-2.5 μm and width of 0.6-0.9 μm. Growth was observed at 10-37 °C with an optimum growth temperature of 28 °C. The pH range for growth was 5.8-8.2 with an optimum pH of 7.0. The most predominant fatty acid was anteiso-C. Under sulfate-reducing conditions, strain SF6 utilized lactate, ethanol and glucose as growth substrate. Chemolithoautotrophic growth on H was not observed, although H was used as electron donor. Fermentative growth occurred on pyruvate. As electron acceptor, sulfate, sulfite, thiosulfate and nitrate supported heterotrophic growth of the strain. The complete genome of strain SF6 is composed of a circular chromosome with length of 3.8 Mbp and G+C content of 54 mol%. Analyses of the 16S rRNA gene and whole genome sequence indicated that strain SF6 belongs to the genus Pseudodesulfovibrio but distinct form all existing species in the genus. On the basis of its genomic and phenotypic properties, strain SF6 (= DSM111931 = NBRC 114895) is proposed as the type strain of a new species, with name of Pseudodesulfovibrio sediminis sp. nov.
一株新的嗜中温和嗜中性硫酸盐还原菌，SF6 菌株，从日本一个半咸水湖的沉积物中分离得到。SF6 菌株的细胞是运动的，呈杆状，长 1.2-2.5μm，宽 0.6-0.9μm。在 10-37°C 下观察到生长，最佳生长温度为 28°C。生长的 pH 范围为 5.8-8.2，最佳 pH 值为 7.0。最主要的脂肪酸是 anteiso-C。在硫酸盐还原条件下，SF6 菌株利用乳酸盐、乙醇和葡萄糖作为生长基质。虽然 H 可用作电子供体，但未观察到 SF6 菌株在化能自养条件下利用 H 进行化学自养生长。丙酮酸可发生发酵生长。硫酸盐、亚硫酸盐、硫代硫酸盐和硝酸盐作为电子受体支持该菌株的异养生长。SF6 菌株的全基因组由一个长度为 3.8 Mbp 的圆形染色体和 54 mol%的 G+C 组成。16S rRNA 基因和全基因组序列分析表明，SF6 菌株属于假脱硫弧菌属，但与该属中所有现有种都不同。基于其基因组和表型特性，SF6 菌株（=DSM111931= NBRC 114895）被提议作为一个新种的模式菌株，命名为 Sediminis 假脱硫弧菌 sp. nov.。
