顺式和反式二氯二氨铂（II）与小牛胸腺细胞核相互作用后的DNA降解
DNA degradation after interaction of cis- and trans-diamminedichloroplatinum (II) with calf thymus nuclei.
作者信息
Oliński R
出版信息
Mol Biol Rep. 1986;11(1):25-8. doi: 10.1007/BF00417591.
PMID:3945232
Abstract
DNA samples isolated from control nuclei and nuclei treated by cis- and trans-diamminedichloroplatinum (DDP) were analyzed by gel electrophoresis. There were no changes in Mr when DNA isolated from nuclei treated with trans-DDP was analyzed. Scans of DNA isolated from nuclei treated with cis-DDP revealed significant changes in Mr. This DNA bears, however, no signs of regular fragmentation. The possible involvement of endonuclease activity in the degradation process is discussed.
摘要
从对照细胞核以及经顺式和反式二氯二氨铂（DDP）处理的细胞核中分离出的DNA样本，通过凝胶电泳进行分析。分析从经反式DDP处理的细胞核中分离出的DNA时，其相对分子质量（Mr）没有变化。对从经顺式DDP处理的细胞核中分离出的DNA进行扫描，发现其相对分子质量有显著变化。然而，这种DNA没有规则片段化的迹象。本文讨论了核酸内切酶活性在降解过程中可能的参与情况。
Zotero 插件