Wing R M, Pjura P, Drew H R, Dickerson R E
EMBO J. 1984 May;3(5):1201-6. doi: 10.1002/j.1460-2075.1984.tb01951.x.
When cisplatin [cis- diamminodichloroplatinum (II)] is diffused into pre-grown crystals of the B-DNA double-helical dodecamer C-G-C-G-A-A-T-T-C-G-C-G, it binds preferentially to the N7 positions of guanines, with what probably is an aquo bridge between Pt and the adjacent O6 atom of the same guanine. The entire guanine ring moves slightly toward the platinum site, into the major groove. Only three of the eight potential cisplatin binding sites on guanines actually are occupied, and this differential reactivity can be explained in terms of the relative freedom of motion of guanines toward the major groove. This shift of guanines upon ligation may weaken the glycosyl bond and assist in the depurination that leads to mismatch SOS repair and G.C. to T.A. transversion.
当顺铂[顺式二氨二氯铂(II)]扩散到B-DNA双螺旋十二聚体C-G-C-G-A-A-T-T-C-G-C-G预先生长的晶体中时，它优先与鸟嘌呤的N7位结合，铂与同一鸟嘌呤相邻的O6原子之间可能形成一个水桥。整个鸟嘌呤环向铂位点轻微移动，进入大沟。鸟嘌呤上八个潜在的顺铂结合位点中实际上只有三个被占据，这种差异反应性可以用鸟嘌呤向大沟移动的相对运动自由度来解释。连接时鸟嘌呤的这种移动可能会削弱糖苷键，并有助于脱嘌呤，从而导致错配SOS修复以及G.C到T.A的颠换。
