Tanner Soraya, Thibault Anna, Leprince Julian Grégoire, Bouillaguet Serge
Division of Cariology and Endodontology, CUMD-University Clinics of Dental Medicine, University of Geneva, 1205 Geneva, Switzerland.
Dent J (Basel). 2024 Sep 27;12(10):308. doi: 10.3390/dj12100308.
: The aim of this study was to evaluate the photothermal effect of a 970 nm diode laser on biofilms. : 72 extracted human single-rooted teeth were prepared, sterilized, and inoculated with to establish a two-week-old biofilm. The specimens were divided into six groups (n = 12): Group 1 (G1)-negative control (PBS-no laser), Group 2 (G2)-positive control (1% NaOCl rinse-no laser), Group 3 (G3)-a 970 nm laser in 1.5 W pulse mode, Group 4 (G4)-a 970 nm laser in 2 W pulse mode, Group 5 (G5)-a 970 nm laser in 1.5 W continuous mode, Group 6 (G6)-a 970 nm laser in 2 W continuous mode. Bacterial viability was evaluated using the LIVE/DEAD BacLight kit and analyzed by flow cytometry (FCM). Temperature changes on the root surface during irradiation were analyzed using a K-type thermocouple. Data were statistically analyzed using one-way ANOVA and Tukey's multiple comparison test (α = 0.05). : Bacterial viability was significantly reduced after laser irradiation in continuous mode using 1.5 W (21% of live bacteria) and 2 W (14% of live bacteria). When the pulsed mode was applied, the reduction in bacterial viability was less, with a mean survival of 53% (1.5 PF, whereas 29% of bacteria survived after 2 W irradiation). : The 970 nm diode laser at 2 W continuous mode effectively reduced the viability of biofilms in root canals without causing unacceptable temperature rises at the root surface.
本研究的目的是评估970nm二极管激光对生物膜的光热效应。制备、灭菌72颗拔除的人单根牙，并接种以建立两周龄的生物膜。将标本分为六组（n = 12）：第1组（G1）-阴性对照（磷酸盐缓冲液-无激光），第2组（G2）-阳性对照（1%次氯酸钠冲洗-无激光），第3组（G3）-1.5W脉冲模式的970nm激光，第4组（G4）-2W脉冲模式的970nm激光，第5组（G5）-1.5W连续模式的970nm激光，第6组（G6）-2W连续模式的970nm激光。使用LIVE/DEAD BacLight试剂盒评估细菌活力，并通过流式细胞术（FCM）进行分析。使用K型热电偶分析照射期间根表面的温度变化。数据采用单因素方差分析和Tukey多重比较检验进行统计学分析（α = 0.05）。连续模式下使用1.5W（活菌的21%）和2W（活菌的14%）激光照射后，细菌活力显著降低。当应用脉冲模式时，细菌活力的降低较少，平均存活率为53%（1.5W），而2W照射后29%的细菌存活。2W连续模式的970nm二极管激光可有效降低根管内生物膜的活力，且不会导致根表面出现不可接受的温度升高。
