Fu Yipeng, Ekambaram Manikandan, Li Kai Chun, Zhang Ya, Cooper Paul R, Mei May Lei
Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9016, New Zealand.
Dent J (Basel). 2024 Oct 8;12(10):323. doi: 10.3390/dj12100323.
Dental caries remains a significant global health problem. One of the fundamental mechanisms underlying the development and progression of dental caries is the dynamic process of demineralisation/remineralisation. In vitro models have played a critical role in advancing our understanding of this process and identifying potential interventions to prevent or arrest dental caries. This literature review aims to provide a structured oversight of in vitro mineralisation models which have been used to study the tooth demineralisation/remineralisation process.
Publications from 2019 to 2023 were screened to identify articles reporting the use of in vitro models to study the demineralisation/remineralisation of tooth caries. The included studies were methodologically assessed for their information on (i) substrate, (ii) lesion formation, and (iii) mineralisation models.
The most reported substrates used in the studies were human teeth along with bovine incisors. Acetic/lactic buffers were the most common solutions to induce caries lesions. pH cycling was the most frequently used mineralisation model for simulating the daily change within the oral environment. This review discussed the advantages and limitations of various approaches.
Standardisation of in vitro mineralisation models is crucial for enabling effective comparison between studies and advancing caries research.
龋齿仍然是一个重大的全球健康问题。龋齿发生和发展的基本机制之一是脱矿质/再矿质化的动态过程。体外模型在增进我们对这一过程的理解以及确定预防或阻止龋齿的潜在干预措施方面发挥了关键作用。本综述旨在对用于研究牙齿脱矿质/再矿质化过程的体外矿化模型进行系统性概述。
筛选2019年至2023年的出版物，以确定报告使用体外模型研究龋齿脱矿质/再矿质化的文章。对纳入研究在以下方面的信息进行方法学评估：(i) 底物，(ii) 病变形成，以及(iii) 矿化模型。
研究中报告最多的底物是人类牙齿和牛切牙。醋酸/乳酸缓冲液是诱导龋损最常用的溶液。pH循环是模拟口腔环境中日常变化最常用的矿化模型。本综述讨论了各种方法的优缺点。
体外矿化模型的标准化对于实现研究之间的有效比较和推进龋齿研究至关重要。
