Lanzino Maria Carolina, Le Long-Quan R V, Höppel Anika, Killinger Andreas, Rheinheimer Wolfgang, Dembski Sofia, Al-Ahmad Ali, Mayr Hermann O, Seidenstuecker Michael
Institute for Manufacturing Technologies of Ceramic Components and Composites (IFKB), University of Stuttgart, 70569 Stuttgart, Germany.
G.E.R.N. Center of Tissue Replacement, Regeneration & Neogenesis, Department of Orthopedics and Trauma Surgery, Faculty of Medicine, Albert-Ludwigs-University of Freiburg, Hugstetter Straße 55, 79106 Freiburg, Germany.
J Funct Biomater. 2024 Sep 25;15(10):281. doi: 10.3390/jfb15100281.
Prosthesis loosening due to lack of osteointegration between an implant and surrounding bone tissue is one of the most common causes of implant failure. Further, bacterial contamination and biofilm formation onto implants represent a serious complication after surgery. The enhancement of osteointegration can be achieved by using bioconductive materials that promote biological responses in the body, stimulating bone growth and thus bonding to tissue. Through the incorporation of antibacterial substances in bioconductive, biodegradable calcium phosphate (CaP) coatings, faster osteointegration and bactericidal properties can be achieved. In this study, Cu-doped CaP supraparticles are spray-dried and suspension-sprayed CaP ceramic coatings with antibacterial properties are prepared using high-velocity suspension flame spraying (HVSFS). The objective was to increase the coatings' porosity and investigate which Cu-doped supraparticles have the strongest antibacterial properties when introduced into the coating layers. Biocompatibility was tested on human Osteosarcoma cells MG63. A porosity of at least 13% was achieved and the supraparticles could be implemented, enhancing it up to 16%. The results showed that the addition of Cu-doped supraparticles did not significantly reduce the number of viable cells compared to the Cu-free sample, demonstrating good biocompatibility. The antimicrobial activity was assessed against the bacterial strains and , with Safe Airborne Antibacterial testing showing a significant reduction in both Gram-positive and Gram-negative strains on the Cu-doped coatings.
由于植入物与周围骨组织之间缺乏骨整合导致的假体松动是植入失败最常见的原因之一。此外，植入物上的细菌污染和生物膜形成是手术后的严重并发症。通过使用促进体内生物反应、刺激骨生长从而与组织结合的生物传导材料，可以增强骨整合。通过在生物传导性、可生物降解的磷酸钙（CaP）涂层中加入抗菌物质，可以实现更快的骨整合和杀菌性能。在本研究中，对铜掺杂的CaP超微粒进行喷雾干燥，并使用高速悬浮火焰喷涂（HVSFS）制备具有抗菌性能的悬浮喷涂CaP陶瓷涂层。目的是增加涂层的孔隙率，并研究将哪些铜掺杂超微粒引入涂层时具有最强的抗菌性能。在人骨肉瘤细胞MG63上测试生物相容性。实现了至少13%的孔隙率，并且可以引入超微粒，将其提高到16%。结果表明，与无铜样品相比，添加铜掺杂超微粒并没有显著减少活细胞数量，表明具有良好的生物相容性。针对细菌菌株 和 评估抗菌活性，安全空气传播抗菌测试表明，铜掺杂涂层上的革兰氏阳性和革兰氏阴性菌株数量均显著减少。
