Komarova Ekaterina G, Sharkeev Yurii P, Sedelnikova Mariya B, Prymak Oleg, Epple Matthias, Litvinova Larisa S, Shupletsova Valeria V, Malashchenko Vladimir V, Yurova Kristina A, Dzyuman Anna N, Kulagina Irina V, Mushtovatova Lyudmila S, Bochkareva Olga P, Karpova Mariia R, Khlusov Igor A
Laboratory of Physics of Nanostructured Biocomposites, Institute of Strength Physics and Materials Science SB RAS, Tomsk 634055, Russia.
Research School of High-Energy Physics, National Research Tomsk Polytechnic University, Tomsk 634050, Russia.
Materials (Basel). 2020 Sep 30;13(19):4366. doi: 10.3390/ma13194366.
This work describes the wettability and biological performance of Zn- and Cu-containing CaP-based coatings prepared by micro-arc oxidation on pure titanium (Ti) and novel Ti-40Nb alloy. Good hydrophilic properties of all the coatings were demonstrated by the low contact angles with liquids, not exceeding 45°. An increase in the applied voltage led to an increase of the coating roughness and porosity, thereby reducing the contact angles to 6° with water and to 17° with glycerol. The free surface energy of 75 ± 3 mJ/m for all the coatings were determined. Polar component was calculated as the main component of surface energy, caused by the presence of strong polar PO and OH bonds. In vitro studies showed that low Cu and Zn amounts (~0.4 at.%) in the coatings promoted high motility of human adipose-derived multipotent mesenchymal stromal cells (hAMMSC) on the implant/cell interface and subsequent cell ability to differentiate into osteoblasts. In vivo study demonstrated 100% ectopic bone formation only on the surface of the CaP coating on Ti. The Zn- and Cu-containing CaP coatings on both substrates and the CaP coating on the Ti-40Nb alloy slightly decreased the incidence of ectopic osteogenesis down to 67%. The MAO coatings showed antibacterial efficacy against and can be arranged as follows: Zn-CaP/Ti > Cu-CaP/TiNb, Zn-CaP/TiNb > Cu-CaP/Ti.
这项工作描述了通过微弧氧化在纯钛（Ti）和新型Ti-40Nb合金上制备的含锌和铜的钙磷基涂层的润湿性和生物学性能。所有涂层均具有良好的亲水性，与液体的接触角较低，不超过45°。施加电压的增加导致涂层粗糙度和孔隙率增加，从而使与水的接触角降低至6°，与甘油的接触角降低至17°。测定了所有涂层的自由表面能为75±3 mJ/m²。极性成分被计算为表面能的主要成分，这是由强极性PO和OH键的存在引起的。体外研究表明，涂层中低含量的铜和锌（约0.4原子%）促进了人脂肪来源的多能间充质基质细胞（hAMMSC）在植入物/细胞界面上的高迁移率以及随后细胞分化为成骨细胞的能力。体内研究表明，仅在钛上的钙磷涂层表面有100%的异位骨形成。两种基材上含锌和铜的钙磷涂层以及Ti-40Nb合金上的钙磷涂层使异位骨形成的发生率略有下降，降至67%。微弧氧化涂层显示出对……的抗菌效果，其排列如下：Zn-CaP/Ti > Cu-CaP/TiNb，Zn-CaP/TiNb > Cu-CaP/Ti。
