Abdollahi Hossein, Amiri Saber, Amiri Farzaneh, Moradi Somayeh, Zarrintaj Payam
Department of Polymer Engineering, Faculty of Engineering, Urmia University, Urmia 57561-51818, Iran.
Department of Food Science and Technology, Faculty of Agriculture, Urmia University, Urmia 57561-51818, Iran.
J Funct Biomater. 2024 Sep 28;15(10):286. doi: 10.3390/jfb15100286.
Designing a wound dressing with controlled uptake, antibacterial, and proper biocompatibility is crucial for the appropriate wound healing process. In this study, alginate/tetracycline (Alg/TC) beads were produced and embedded into chitosan/pluronic/agarose semi-interpenetrating polymer network hydrogel, which serves as a potential biocompatible dressing for treating skin wounds. The effect of pluronic content on the porosity, swelling, mechanical characteristics, and degradation of the hydrogel was investigated. Furthermore, the impact of Alg beads on TC release was subsequently examined. In the absence of Alg beads, faster release was observed. However, after incorporating beads into the hydrogels, the release was sustained. Particularly, the hydrogel containing Alg beads exhibited a nearly linear release, reaching 74% after 2 days in acidic media. The antimicrobial activity and biocompatibility of the hydrogel were also evaluated to assess the capability of the TC-loaded hydrogels for wound dressing applications. The hydrogel demonstrated efficient antibacterial features against Gram-positive and Gram-negative bacteria. Additionally, the sample behavior was evaluated against exposure to yeast. Furthermore, based on biocompatibility studies using HFF2 cells, the TC-loaded hydrogel exhibited remarkable biocompatibility. Overall, this novel composite hydrogel shows remarkable biocompatibility and antibacterial activities which can be used as a great potential wound dressing to prevent wound infections due to its effective inhibition of bacterial growth.
设计一种具有可控吸收、抗菌和适当生物相容性的伤口敷料对于适当的伤口愈合过程至关重要。在本研究中，制备了藻酸盐/四环素（Alg/TC）珠，并将其嵌入壳聚糖/普朗尼克/琼脂糖半互穿聚合物网络水凝胶中，该水凝胶可作为治疗皮肤伤口的潜在生物相容性敷料。研究了普朗尼克含量对水凝胶孔隙率、溶胀、力学特性和降解的影响。此外，随后研究了Alg珠对TC释放的影响。在没有Alg珠的情况下，观察到更快的释放。然而，将珠子加入水凝胶后，释放得以持续。特别是，含有Alg珠的水凝胶呈现出近乎线性的释放，在酸性介质中2天后释放率达到74%。还评估了水凝胶的抗菌活性和生物相容性，以评估负载TC的水凝胶在伤口敷料应用中的能力。该水凝胶对革兰氏阳性菌和革兰氏阴性菌均表现出有效的抗菌特性。此外，还评估了样品对酵母暴露的反应。此外，基于使用HFF2细胞的生物相容性研究，负载TC的水凝胶表现出显著的生物相容性。总体而言，这种新型复合水凝胶具有显著的生物相容性和抗菌活性，由于其对细菌生长的有效抑制，可作为一种极具潜力的伤口敷料来预防伤口感染。
