Fu Zhizhen, Chen Yanjie, Cai Gaolei, Peng Huijuan, Wang Xiaoyu, Li Ping, Gu Aiguo, Li Yanli, Ma Dongfang
Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, College of Agriculture, Yangtze University, Jingzhou 434025, China.
Shiyan Academy of Agricultural Sciences, Shiyan 442000, China.
J Fungi (Basel). 2024 Oct 3;10(10):692. doi: 10.3390/jof10100692.
Fusarium head blight (FHB), primarily caused by , is a devastating crop disease that leads to significant declines in wheat yield and quality worldwide. Long non-coding RNAs (lncRNAs) are found to play significant functions in various biological processes, but their regulatory functions in the sexual reproduction and pathogenicity of have not been studied extensively. This study identified an antisense lncRNA, named lncRsn, located in the transcription initiation site region between the 5'-flanking gene and the 3'-flanking gene . A deletion mutant of lncRsn (ΔlncRsn) was constructed through homologous recombination. ΔlncRsn exhibited huge reductions in pathogen and sexual reproduction. Additionally, the deletion of lncRsn disrupted the biosynthesis of deoxynivalenol (DON) and impaired the formation of infection structures. RT-qPCR analysis reveals that lncRsn may negatively regulate the transcription of the target gene FgSna. This study found that lncRsn plays an important role in sexual and asexual reproduction, pathogenicity, virulence, osmotic stress, and cell wall integrity (CWI) in . Further characterization of pathogenesis-related genes and the reaction between lncRsn and protein-coding genes will aid in developing novel approaches for controlling diseases.
镰刀菌穗腐病（FHB）主要由 引起，是一种毁灭性的作物病害，导致全球小麦产量和质量大幅下降。长链非编码RNA（lncRNAs）在各种生物过程中发挥着重要作用，但其在 的有性生殖和致病性中的调控功能尚未得到广泛研究。本研究鉴定了一种反义lncRNA，命名为lncRsn，位于5'侧翼基因 和3'侧翼基因 之间的转录起始位点区域。通过同源重组构建了lncRsn的缺失突变体（ΔlncRsn）。ΔlncRsn在病原体和有性生殖方面表现出大幅减少。此外，lncRsn的缺失破坏了脱氧雪腐镰刀菌烯醇（DON）的生物合成，并损害了感染结构的形成。RT-qPCR分析表明，lncRsn可能对靶基因FgSna的转录起负调控作用。本研究发现lncRsn在 的有性和无性生殖、致病性、毒力、渗透胁迫和细胞壁完整性（CWI）中起重要作用。进一步表征与发病机制相关的基因以及lncRsn与蛋白质编码基因之间的相互作用，将有助于开发控制 疾病的新方法。
