Myrtsi Eleni D, Koulocheri Sofia D, Iliopoulos Vassilios, Haroutounian Serkos A
Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.
Antioxidants (Basel). 2021 Jul 23;10(8):1174. doi: 10.3390/antiox10081174.
The well-established, health-benefitting effects of grapevines and derivatives (wines and vinification byproducts) are attributed to their antioxidant phenolic content. The dearth of an efficient method for the simultaneous quantitation of antioxidant phenolics prompted us to develop a novel method utilizing triple quadrupole LC-MS/MS for the accurate, fast, simultaneous quantitation of the 32 most abundant grapevine phenolics. The fully validated, novel method is capable to simultaneously record the quantitative presence of 12 phenolic acids, 19 polyphenols and coniferyl aldehyde (a phenolic compound extracted from cork stoppers into wines) and is applicable for the determination of antioxidant phenolics content of grape berries, pomace, stems and wines. Its utility was demonstrated for three native Greek grapevine varieties, two red (Mandilaria and Aidani mavro) and one white (Monemvassia). Results herein highlighted the stems of the Monemvassia white variety as particularly rich in antioxidant phenolics such as the flavonol monomer (+)-catechin (387 mg/kg) and the dimer procyanidin B1 (400 mg/kg) along with stilbene phytoalexin -resveratrol (24 mg/kg). These results are in line with the TPC, TFC and TTC content of stems and the determined antioxidant capacities, highlighting the stems of this variety as potentially exploitable source of antioxidant phenolics.
葡萄及其衍生物（葡萄酒和酿酒副产物）对健康有益的既定功效归因于其抗氧化酚类物质含量。由于缺乏一种能同时定量抗氧化酚类物质的有效方法，促使我们开发一种利用三重四极杆液相色谱 - 串联质谱（LC-MS/MS）的新方法，用于准确、快速地同时定量32种最丰富的葡萄酚类物质。这种经过充分验证的新方法能够同时记录12种酚酸、19种多酚和松柏醛（一种从软木塞中提取到葡萄酒中的酚类化合物）的定量存在情况，适用于测定葡萄浆果、果渣、茎和葡萄酒中的抗氧化酚类物质含量。我们通过三个希腊本土葡萄品种（两个红色品种：曼迪利亚和阿伊达尼马夫罗，以及一个白色品种：莫内瓦西亚）证明了该方法的实用性。本文的结果突出表明，莫内瓦西亚白色品种的茎尤其富含抗氧化酚类物质，如黄酮醇单体（+）-儿茶素（387毫克/千克）和二聚体原花青素B1（400毫克/千克）以及芪类植物抗毒素白藜芦醇（24毫克/千克）。这些结果与茎的总酚含量（TPC）、总黄酮含量（TFC）和总单宁含量（TTC）以及测定的抗氧化能力一致，突出了该品种的茎作为抗氧化酚类物质潜在可利用来源的价值。
