Multidisciplinary Laboratory of Food and Health (LabMAS), School of Applied Sciences (FCA), Universidade Estadual de Campinas, São Paulo 13083-871, Brazil.
Institute of Food Science Research (CIAL-CSIC), Nicolás Cabrera 9, Campus UAM Cantoblanco, 28049 Madrid, Spain.
Int J Mol Sci. 2024 Sep 17;25(18):9992. doi: 10.3390/ijms25189992.
Pomegranate ( L.) peel is a potential source of bioactive phenolic compounds such as ellagic acid and α- and β-punicalagin. This work explores the efficiency of natural deep eutectic solvents combined with ultrasound-assisted extraction (UAE) and pressurized liquid extraction (PLE) for their extraction. Five NaDESs were evaluated by employing UAE (25 °C, for 50 min) to determine their total phenolic content (Folin-Ciocalteu assay) and ellagic acid and α- and β-punicalagin contents (high-performance liquid chromatography (HPLC-DAD)). The NaDES composed of choline chloride (ChCl) and glycerol (Gly) (1:2, molar ratio) was the most efficient in the UAE when compared with the rest of the NaDESs and water extracts. Therefore, ChCl:Gly was further evaluated using PLE at different temperatures (40, 80, 120 and 160 °C). The PLE-NaDES extract obtained at 80 °C for 20 min at 1500 psi exhibited the highest contents of ellagic acid and α- and β-punicalagin compared to the rest of the temperatures and PLE-water extracts obtained under the same extraction conditions. Combining UAE or PLE with a NaDES emerges as a sustainable alternative for extracting ellagic acid and α- and β-punicalagin from pomegranate peel.
石榴皮是生物活性酚类化合物（如鞣花酸和α-和β-鞣花单宁）的潜在来源。本工作探索了天然深共晶溶剂结合超声辅助提取（UAE）和加压液体提取（PLE）的提取效率。通过 UAE（25°C，50 分钟）评估了 5 种 NaDES 的总酚含量（福林-肖卡尔特法）和鞣花酸及α-和β-鞣花单宁含量（高效液相色谱法（HPLC-DAD））。与其他 NaDES 和水提取物相比，由氯化胆碱（ChCl）和甘油（Gly）（1:2，摩尔比）组成的 NaDES 在 UAE 中效率最高。因此，进一步在不同温度（40、80、120 和 160°C）下使用 PLE 评估 ChCl:Gly。与其他温度和在相同提取条件下获得的 PLE-水提取物相比，在 80°C 下以 1500 psi 提取 20 分钟得到的 PLE-NaDES 提取物中鞣花酸和α-和β-鞣花单宁含量最高。将 UAE 或 PLE 与 NaDES 结合起来，是从石榴皮中提取鞣花酸和α-和β-鞣花单宁的可持续替代方法。
