Department of Earth, Environment and Life Sciences (DISTAV), University of Genova, Via Pastore 3, 16132 Genova, Italy.
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 10 Pardubice, Czech Republic.
Mar Drugs. 2024 Oct 12;22(10):468. doi: 10.3390/md22100468.
The venom contained within cnidarian nematocysts has a complex composition and holds significant potential for biotechnological applications. In this context, one of the most effective methods for studying nematocyst contents is the proteomic approach, which can detect even trace amounts of compounds while minimizing the need for large-scale animal collection, thus helping to preserve ecosystem integrity. This study aimed to provide a comprehensive proteomic and biochemical characterization of the crude nematocyst extract from the common hydrozoan . Despite not being harmful to humans, the analysis of the crude venom extract from brought to the identification of 783 different proteins, categorized into structural components, enzymes, and potential toxins, revealing a qualitative composition of the venom similar to that of other more toxic cnidarians. Biochemical assays confirmed the presence of various active hydrolytic enzymes within the extract, including proteases, phospholipases, hyaluronidases, DNases, and chitinases. These findings pave the road for future studies involving the pharmacological applications of venom components through recombinant production and functional testing.
刺胞动物的刺丝囊毒液成分复杂，具有巨大的生物技术应用潜力。在这方面，研究刺丝囊内容物最有效的方法之一是蛋白质组学方法，它可以检测到痕量的化合物，同时最大限度地减少对大规模动物采集的需求，从而有助于保护生态系统的完整性。本研究旨在对常见水螅水母的刺丝囊粗提物进行全面的蛋白质组学和生化特性分析。尽管对人类没有危害，但从粗毒液提取物的分析中鉴定出了 783 种不同的蛋白质，分为结构成分、酶和潜在毒素，揭示了毒液的定性组成与其他毒性更强的刺胞动物相似。生化分析证实提取物中存在多种活性水解酶，包括蛋白酶、磷脂酶、透明质酸酶、DNase 和几丁质酶。这些发现为通过重组生产和功能测试来研究水母毒液成分的药理学应用铺平了道路。
