State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Simon F.S. Li Marine Science Laboratory, State Key Laboratory of Agrobiotechnology, School of Life Sciences, The Chinese University of Hong Kong, Hong Kong, China.
Mar Drugs. 2020 Dec 21;18(12):655. doi: 10.3390/md18120655.
Venomics, the study of biological venoms, could potentially provide a new source of therapeutic compounds, yet information on the venoms from marine organisms, including cnidarians (sea anemones, corals, and jellyfish), is limited. This study identified the putative toxins of two species of jellyfish-edible jellyfish Kishinouye, 1891, also known as flame jellyfish, and Amuska jellyfish Goette, 1886. Utilizing nano-flow liquid chromatography tandem mass spectrometry (nLC-MS/MS), 3000 proteins were identified from the nematocysts in each of the above two jellyfish species. Forty and fifty-one putative toxins were identified in and , respectively, which were further classified into eight toxin families according to their predicted functions. Amongst the identified putative toxins, hemostasis-impairing toxins and proteases were found to be the most dominant members (>60%). The present study demonstrates the first proteomes of nematocysts from two jellyfish species with economic and environmental importance, and expands the foundation and understanding of cnidarian toxins.
毒液学是研究生物毒液的学科，它可能为治疗化合物提供新的来源，但关于海洋生物毒液的信息，包括刺胞动物（海葵、珊瑚和水母），是有限的。本研究鉴定了两种水母——可食用的水母 Kishinouye，1891 年，也被称为火焰水母，和 Amuska 水母 Goette，1886 年的潜在毒素。利用纳流液相色谱串联质谱（nLC-MS/MS），从上述两种水母的刺丝囊中分别鉴定出 3000 种蛋白质。在 和 中分别鉴定出 40 种和 51 种潜在毒素，根据其预测功能进一步分为 8 个毒素家族。在鉴定出的潜在毒素中，发现止血功能障碍毒素和蛋白酶是最主要的成员（>60%）。本研究首次展示了两种具有经济和环境重要性的水母刺丝囊的蛋白质组，扩展了刺胞动物毒素的基础和认识。
