Department of Respiratory, the Second People's Hospital of Lanzhou, Lanzhou, China.
Department of Infectious, the Second People's Hospital of Lanzhou, Lanzhou, China.
Bull Exp Biol Med. 2024 Oct;177(6):780-786. doi: 10.1007/s10517-024-06267-w. Epub 2024 Oct 26.
Serine-threonine kinase receptor-associated protein (STRAP) regulates cell proliferation and apoptosis by binding to many target proteins and plays an important regulatory role in tumor development. We studied the effects of STRAP on non-small cell lung cancer (NSCLC) in vivo and in vitro in order to elucidate possible mechanisms underlying the regulatory effects of this protein. The levels of STRAP in NSCLC tissues and cells were determined by quantitative reverse transcription PCR, immunohistochemical staining, and Western blotting. In in vitro experiments, A549 and HCC827 cells were transfected with small interfering RNA (siRNA) to knockdown STRAP (si-STRAP) or with negative control sequence; cell migration and invasion were detected by scratch and Transwell assays, respectively. The expression levels of X-linked inhibitor of apoptosis protein (XIAP), caspase-3, and caspase-9 were determined by Western blotting. In addition, we analyzed changes of tumor volume in a nude mouse NSCLC model. STRAP was highly expressed in NSCLC tissues and cells, but its expression was significantly suppressed in A549 and HCC827 cells transfected with si-STRAP. STRAP knockdown resulted in a significant inhibition of migration and invasion of A549 and HCC827 cells. It also significantly reduced the expression of XIAP and elevated expression of caspase-3 and caspase-9. In nude mice with tumor originated from transplanted A549 cells, inhibition of STRAP expression retarded the tumor growth. Overall, these findings indicate that STRAP is overexpressed in NSCLC, while knockdown of STRAP gene inhibits the growth of NSCLC.
丝氨酸-苏氨酸激酶受体相关蛋白（STRAP）通过与许多靶蛋白结合来调节细胞增殖和凋亡，在肿瘤发生发展中发挥重要的调节作用。我们研究了 STRAP 在体内和体外对非小细胞肺癌（NSCLC）的影响，以阐明该蛋白调节作用的可能机制。通过定量逆转录 PCR、免疫组织化学染色和 Western blot 检测 NSCLC 组织和细胞中 STRAP 的水平。在体外实验中，用小干扰 RNA（siRNA）转染 A549 和 HCC827 细胞以敲低 STRAP（si-STRAP）或阴性对照序列；划痕和 Transwell 实验分别检测细胞迁移和侵袭；Western blot 检测 X 连锁凋亡抑制蛋白（XIAP）、caspase-3 和 caspase-9 的表达水平。此外，我们分析了裸鼠 NSCLC 模型中肿瘤体积的变化。STRAP 在 NSCLC 组织和细胞中高表达，但在转染 si-STRAP 的 A549 和 HCC827 细胞中其表达显著受到抑制。STRAP 敲低导致 A549 和 HCC827 细胞迁移和侵袭显著抑制。它还显著降低了 XIAP 的表达，同时上调了 caspase-3 和 caspase-9 的表达。在来源于移植 A549 细胞的裸鼠肿瘤中，抑制 STRAP 表达可减缓肿瘤生长。总的来说，这些发现表明 STRAP 在 NSCLC 中过表达，而 STRAP 基因敲低抑制 NSCLC 的生长。
