AgroParisTech, GQE-Le Moulon, PAPPSO, Université Paris-Saclay, INRAE, CNRS, 91190 Gif-sur-Yvette, France.
LEPSE, INRAE, Montpellier SupAgro, Université Montpellier, 34293 Montpellier, France.
Biomolecules. 2024 Sep 30;14(10):1239. doi: 10.3390/biom14101239.
Water deficit is a major cause of yield loss for maize (), leading to ovary abortion when applied at flowering time. To help understand the mechanisms involved in this phenomenon, the proteome response to water deficit has been analysed in developing ovaries at the silk emergence stage and five days later. Differential analysis, abundance pattern clustering and co-expression networks were performed in order to draw a general picture of the proteome changes all along ovary development and under the effect of water deficit. The results show that even mild water deficit has a major impact on ovary proteome, but this impact is very different from a response to stress. A part of the changes can be related to a slowdown of ovary development, while another part cannot. In particular, ovaries submitted to water deficit show an increase in proteins involved in protein biosynthesis and in vesicle transport together with a decrease in proteins involved in amino acid metabolism and proteolysis. According to the functions of increased proteins, the changes may be linked to auxin, brassinosteroids and jasmonate signalling but not abscisic acid.
水分亏缺是玉米（）产量损失的主要原因，在开花期施用会导致子房败育。为了帮助理解这一现象的机制，在花丝出现阶段和五天后对发育中的子房进行了水分亏缺下的蛋白质组响应分析。为了全面了解整个子房发育过程中和水分亏缺下蛋白质组的变化情况，进行了差异分析、丰度模式聚类和共表达网络分析。结果表明，即使是轻度的水分亏缺也会对子房蛋白质组产生重大影响，但这种影响与应激反应非常不同。一部分变化可能与子房发育的减缓有关，而另一部分则不能。特别是，水分亏缺处理的子房中参与蛋白质生物合成和小泡运输的蛋白质增加，而参与氨基酸代谢和蛋白水解的蛋白质减少。根据增加的蛋白质的功能，这些变化可能与生长素、油菜素内酯和茉莉酸信号转导有关，但与脱落酸无关。
