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基于蛋白质的仿生方法制备的六种提取物的化学成分与抗氧化活性

Chemical Composition and Antioxidant Activity of Six Extracts Using Protein-Based Biomimetic Methods.

作者信息

Barbu Ioana Andreea, Toma Vlad Alexandru, Moț Augustin Cătălin, Vlase Ana-Maria, Butiuc-Keul Anca, Pârvu Marcel

机构信息

Faculty of Biology and Geology, Babeș-Bolyai University, 1, M. Kogălniceanu Street, 400084 Cluj-Napoca, Romania.

Doctoral School of Integrative Biology, Babeș-Bolyai University, 400015 Cluj-Napoca, Romania.

出版信息

Antioxidants (Basel). 2024 Sep 29;13(10):1182. doi: 10.3390/antiox13101182.

Abstract

Medicinal plants are a valuable reservoir of novel pharmacologically active compounds. ROS and free radicals are primary contributors to oxidative stress, a condition associated with the onset of degenerative diseases such as cancer, coronary heart disease, and vascular disease. In this study, we used different spectrophotometry methods to demonstrate the antioxidant properties of 6 extracts: ; ; : Arieș red cultivar of , and white variety of ; ; and subsp. . HPLC-MS determined the chemical composition of the extracts. Among the tested extracts, the Arieș red cultivar of stands out as having the best antioxidant activity, probably due to the high content of polyphenols and alliin (12.67 µg/mL and 3565 ng/mL, respectively). The results obtained in this study show that extracts have antioxidant activity, but also free radical scavenging capabilities. Also, their interactions with cytochrome c and hemoglobin can be the basis of future studies to create treatments for oxidative stress-related diseases.

摘要

药用植物是新型药理活性化合物的宝贵来源。活性氧(ROS)和自由基是氧化应激的主要促成因素,氧化应激是一种与癌症、冠心病和血管疾病等退行性疾病的发病相关的状况。在本研究中,我们使用不同的分光光度法来证明6种提取物的抗氧化特性:;;:的阿里什红品种,以及的白色品种;;和亚种。高效液相色谱-质谱联用(HPLC-MS)测定了提取物的化学成分。在测试的提取物中,的阿里什红品种表现出最佳的抗氧化活性,这可能归因于其多酚和蒜氨酸的高含量(分别为12.67μg/mL和3565ng/mL)。本研究获得的结果表明,提取物具有抗氧化活性,同时也具有自由基清除能力。此外,它们与细胞色素c和血红蛋白的相互作用可能成为未来研究开发与氧化应激相关疾病治疗方法的基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8795/11504208/168429ead696/antioxidants-13-01182-g001.jpg

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