Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250103, China.
School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China.
Int J Mol Sci. 2024 Oct 16;25(20):11097. doi: 10.3390/ijms252011097.
Recently, there has been a growing interest in the biocontrol activity of volatile organic compounds (VOCs) produced by microorganisms. This study specifically focuses on the effects of VOCs produced by the co-culture of B418 and T11-W for the control of two phytopathogenic fungi, and f. sp. Owen. The antagonistic activity of VOCs released in mono- and co-culture modes was evaluated by inhibition assays on a Petri dish and in detached fruit experiments, with the co-culture demonstrating significantly higher inhibitory effects on the phytopathogens on both the plates and fruits compared with the mono-cultures. Metabolomic profiles of VOCs were conducted using the solid-liquid microextraction technique, revealing 341 compounds with significant changes in their production during the co-culture. Among these compounds, linalool, dimethyl trisulfide, dimethyl disulfide, geranylacetone, 2-phenylethanol, and acetophenone were identified as having strong antagonistic activity through a standard inhibition assay. These key compounds were found to be related to the improved inhibitory effect of the B418 and T11-W co-culture. Overall, the results suggest that VOCs produced by the co-culture of B418 and T11-W possess great potential in biological control.
最近，微生物产生的挥发性有机化合物（VOCs）的生物防治活性引起了越来越多的关注。本研究特别关注 B418 和 T11-W 共培养物产生的 VOCs 对两种植物病原菌 和 f. sp. Owen 的控制作用。通过在培养皿和离体果实实验中的抑制试验评估了单培养和共培养物中释放的 VOCs 的拮抗活性，与单培养相比，共培养对病原菌在平板和果实上的抑制作用显著更高。使用固-液微萃取技术进行 VOCs 的代谢组学分析，揭示了 341 种化合物在共培养过程中其产量有显著变化。在这些化合物中，通过标准抑制试验鉴定出芳樟醇、二甲基三硫化物、二甲基二硫化物、香叶基丙酮、2-苯乙醇和苯乙酮具有很强的拮抗活性。这些关键化合物与 B418 和 T11-W 共培养物抑制效果的提高有关。总的来说，结果表明 B418 和 T11-W 共培养物产生的 VOCs 在生物防治方面具有巨大的潜力。
