Rinaldi Valentina, Piscitelli Fabiana, Boari Andrea, Verde Roberta, Crisi Paolo Emidio, Bisogno Tiziana
Department of Veterinary Medicine, Veterinary Teaching Hospital, University of Teramo, 64100 Teramo, Italy.
Institute of Biomolecular Chemistry (ICB)-CNR, 34, 80078 Pozzuoli, Italy.
Animals (Basel). 2024 Oct 16;14(20):2986. doi: 10.3390/ani14202986.
A cutaneous mast cell tumor (cMCT) is among the most common tumors in dogs. Endocannabinoids (eCBs) belong to the endocannabinoid system (ECS), which involves also cannabinoid receptors and an enzymatic system of biosynthesis and degradation. In this study, plasma levels of -arachidonoylethanolamine (AEA), 2-arachidonoylglycerol (2-AG), -palmitoylethanolamine (PEA), and -oleoylethanolamine (OEA) were evaluated in 17 dogs with MCTs of varying histological grades and clinical stages, as well as in a control group of 11 dogs. Dogs affected by cMCT had higher plasma levels of 2-AG ( = 0.0001) and lower levels of AEA ( = 0.0012) and PEA ( = 0.0075) compared to the control group, while no differences were observed at the OEA level between healthy and cMCT dogs ( = 0.9264). The ability of eCBs to help discriminate between healthy and cMCT dogs was interrogated through the area under the ROC curve (AUC). An accuracy of 0.98 (95% confidence interval [CI], 0.94-1.02) was found for 2-AG, of 0.85 (95% CI, 0.71-0.99) for AEA, and of 0.81% for PEA (95% CI, 0.64-0.69). Values > 52.75 pmol/mL for 2-AG showed 94% sensitivity and 90% specificity in distinguishing cMCT. This is the first study to demonstrate alterations in plasmatic levels of eCBs in dogs affected by MCTs, suggesting the significance of these biomarkers in the tumorigenic process and their potential use as biomarkers in the future.
皮肤肥大细胞瘤（cMCT）是犬类中最常见的肿瘤之一。内源性大麻素（eCBs）属于内源性大麻素系统（ECS），该系统还涉及大麻素受体以及生物合成和降解的酶系统。在本研究中，对17只组织学分级和临床分期各异的患有MCT的犬以及11只犬的对照组进行了血浆中花生四烯酸乙醇胺（AEA）、2-花生四烯酸甘油酯（2-AG）、棕榈酸乙醇胺（PEA）和油酸乙醇胺（OEA）水平的评估。与对照组相比，患有cMCT的犬血浆中2-AG水平较高（P = 0.0001），AEA（P = 0.0012）和PEA（P = 0.0075）水平较低，而健康犬和cMCT犬之间在OEA水平上未观察到差异（P = 0.9264）。通过ROC曲线下面积（AUC）来研究eCBs区分健康犬和cMCT犬的能力。发现2-AG的准确度为0.98（95%置信区间[CI]，0.94 - 1.02），AEA为0.85（95%CI，0.71 - 0.99），PEA为0.81（95%CI，0.64 - 0.69）。2-AG值>52.75 pmol/mL在区分cMCT时显示出94%的敏感性和90%的特异性。这是第一项证明受MCT影响的犬血浆中eCBs水平发生改变的研究，表明这些生物标志物在肿瘤发生过程中的重要性以及它们未来作为生物标志物的潜在用途。
