Luo Wei, Tan Qisong, Li Hui, Ye Tao, Xiao Tao, Tian Xingzhou, Wang Weiwei
Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China.
College of Animal Science, Guizhou University, Guiyang 550025, China.
Animals (Basel). 2024 Oct 18;14(20):3020. doi: 10.3390/ani14203020.
This study was conducted to investigate the effects of different levels of on the performance, egg quality, serum immune and antioxidant indices, and cecal microflora of 300-day-old Chishui black-bone chickens during the peak laying period. A total of 360 Chishui black-bone chickens were selected as the experimental animals. They were randomly allocated into four groups: the control group (CON), trial group I (T1), trial group II (T2), and trial group III (T3), each group with six replicates and 15 hens in each replicate. The control group was fed a basal diet, and the experimental groups were fed a basal diet supplemented with 0.8%, 1.6%, and 2.4% , respectively. The accommodation period was 14 d, and the experimental period was 60 d. The statistical software SPSS was used to perform a one-way analysis of variance (ANOVA) on the experimental data, and Duncan's method was used to perform multiple comparisons among groups. The results showed the following: compared with those of the control group, the average daily gain of the laying hens significantly decreased in the 1.6% group ( < 0.05); adding significantly reduced the content of malondialdehyde in the serum ( < 0.05), and the addition of 0.8% tea leaves significantly increased the immunoglobulin M and immunoglobulin A contents ( < 0.05); the egg yolk weight, eggshell thickness, eggshell strength, and yolk color of the laying hens significantly decreased in the 1.6% group ( < 0.05), and the addition of at the level of 2.4% significantly increased the percentage of umami, essential, and total amino acids ( < 0.05); and the structure of intestinal microorganisms was improved, and the abundance of Bacteroidetes and Bacteroidaceae significantly increased, while the abundance of Firmicutes and Lachnospiraceae significantly decreased ( < 0.05).
本研究旨在探讨不同水平的[具体物质未给出]对300日龄赤水乌骨鸡产蛋高峰期生产性能、蛋品质、血清免疫和抗氧化指标以及盲肠微生物区系的影响。共选取360只赤水乌骨鸡作为实验动物。它们被随机分为四组：对照组（CON）、实验组I（T1）、实验组II（T2）和实验组III（T3），每组6个重复，每个重复15只母鸡。对照组饲喂基础日粮，实验组分别饲喂添加0.8%、1.6%和2.4%[具体物质未给出]的基础日粮。预试期为14天，试验期为60天。使用统计软件SPSS对实验数据进行单因素方差分析（ANOVA），并采用Duncan法进行组间多重比较。结果表明：与对照组相比，1.6%[具体物质未给出]组蛋鸡的平均日增重显著降低（P<0.05）；添加[具体物质未给出]显著降低了血清中丙二醛的含量（P<0.05），添加0.8%茶叶显著提高了免疫球蛋白M和免疫球蛋白A的含量（P<0.05）；1.6%[具体物质未给出]组蛋鸡的蛋黄重、蛋壳厚度、蛋壳强度和蛋黄颜色显著降低（P<0.05），添加2.4%水平的[具体物质未给出]显著提高了鲜味、必需和总氨基酸的百分比（P<0.05）；肠道微生物结构得到改善，拟杆菌门和拟杆菌科的丰度显著增加，而厚壁菌门和毛螺菌科的丰度显著降低（P<0.05）。
