Tyagi Anuradha, Kumar Vinay, Joshi Navneet, Dhingra Harish Kumar
Department of Biosciences, School of Liberal Arts and Sciences, Mody University of Science and Technology, Lakshmangarh 332311, Rajasthan, India.
Department of Medicine, Pennsylvania State University, Hershey Medical Center, Hershey, PA 17033, USA.
Microorganisms. 2024 Sep 27;12(10):1956. doi: 10.3390/microorganisms12101956.
is a biofilm-forming bacterium responsible for various human infections, one particularly challenging to treat due to its antibiotic resistance. Biofilms can form on both soft tissues and medical devices, leading to persistent and hard-to-treat infections. Combining multiple antimicrobials is a potential approach to overcoming this resistance. This study explored the effects of ursodeoxycholic acid (UDCA) combined with the antibiotic ciprofloxacin against biofilms, aiming to evaluate any synergistic effects. Results showed that UDCA and ciprofloxacin co-treatment significantly reduced biofilm formation and disrupted pre-formed biofilms more effectively than either agent alone ( < 0.01). The combination also displayed a slight synergistic effect, with a fractional inhibitory concentration of 0.65. Additionally, the treatment reduced the production of extracellular polymeric substances, increased reactive oxygen species production, decreased metabolic activity, altered cell membrane permeability, and lowered cell surface hydrophobicity in . Furthermore, it diminished biofilm-associated pathogenic factors, including proteolytic activity and staphyloxanthin production. Overall, the UDCA-ciprofloxacin combination shows considerable promise as a strategy to combat infections related to staphylococcal biofilms, offering a potential solution to the healthcare challenges posed by antibiotic-resistant .
是一种形成生物膜的细菌，可导致多种人类感染，因其抗生素耐药性，其中一种感染特别难以治疗。生物膜可在软组织和医疗设备上形成，导致持续性且难以治疗的感染。联合使用多种抗菌药物是克服这种耐药性的一种潜在方法。本研究探讨了熊去氧胆酸（UDCA）与抗生素环丙沙星联合对生物膜的影响，旨在评估是否存在协同效应。结果表明，与单独使用任何一种药物相比，UDCA和环丙沙星联合治疗能更显著地减少生物膜形成，并更有效地破坏预先形成的生物膜（<0.01）。该组合还显示出轻微的协同效应，部分抑菌浓度为0.65。此外，该治疗降低了细胞外聚合物的产生，增加了活性氧的产生，降低了代谢活性，改变了细胞膜通透性，并降低了的细胞表面疏水性。此外，它减少了与生物膜相关的致病因素，包括蛋白水解活性和金黄色葡萄球菌素的产生。总体而言，UDCA-环丙沙星组合作为对抗与葡萄球菌生物膜相关感染的策略显示出相当大的前景，为抗生素耐药性带来的医疗挑战提供了一个潜在的解决方案。
