Zheng Xiaoting, Chen Qiuyu, Liang Xueying, Kumar Vikash, Loor Alfredo, Dong Hongbiao, Liu Chang, Yang Jinlong, Zhang Jiasong
Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China.
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Microorganisms. 2024 Oct 5;12(10):2020. doi: 10.3390/microorganisms12102020.
The bullfrog is one of the main farmed frog species in China, with a low overall survival of farmed bullfrogs from hatching to harvest since bullfrog tadpoles are fragile during the metamorphosis period. The intestinal bacterial community can play crucial roles in animal development; however, little is known about the alteration of the gut microbial community of during metamorphosis. The present study used 16S rRNA amplicon sequencing to investigate the intestinal bacterial community in at four distinct developmental stages. Moreover, we determined the bullfrog's body morphological parameters and the intestine histology at different developmental stages. The results showed a reduction in the total length and snout-vent length of during metamorphosis. The intestinal microbial composition of exhibited variation throughout the process of metamorphosis. The terrestrial stage showed shifts in the bacterial composition compared to the aquatic stages, including a reduction in and an increase in . Furthermore, the presence of , , , , , , , and in metamorphosis appears to be mainly related to the host's epithelial cells' height and total body mass. The results indicated that the intestinal microbial composition changed with the bullfrog-tadpole metamorphosis. The genera of , , , , , , , and might be potential probiotics.
牛蛙是中国主要的养殖蛙类之一，由于牛蛙蝌蚪在变态期较为脆弱，养殖牛蛙从孵化到收获的总体存活率较低。肠道细菌群落可在动物发育中发挥关键作用；然而，对于变态过程中肠道微生物群落的变化知之甚少。本研究采用16S rRNA扩增子测序技术，调查了牛蛙在四个不同发育阶段的肠道细菌群落。此外，我们还测定了不同发育阶段牛蛙的身体形态参数和肠道组织学。结果显示，牛蛙在变态过程中全长和吻肛长减小。牛蛙的肠道微生物组成在整个变态过程中表现出变化。与水生阶段相比，陆生阶段的细菌组成发生了变化，包括嗜水气单胞菌减少和不动杆菌增加。此外，嗜水气单胞菌、不动杆菌、芽孢杆菌属、葡萄球菌属、乳杆菌属、柠檬酸杆菌属、假单胞菌属和肠球菌属在牛蛙变态过程中的存在似乎主要与宿主上皮细胞高度和总体重有关。结果表明，肠道微生物组成随牛蛙蝌蚪变态而变化。嗜水气单胞菌属、不动杆菌属、芽孢杆菌属、葡萄球菌属、乳杆菌属、柠檬酸杆菌属、假单胞菌属和肠球菌属可能是潜在的益生菌。
