Zea Miguel, Ben Halima Hamdi, Villa Rosa, Nemeir Imad Abrao, Zine Nadia, Errachid Abdelhamid, Gabriel Gemma
Instituto de Microelectrónica de Barcelona IMB-CNM (CSIC), Campus Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain.
Institut UTINAM, UMR CNRS 6213, Université de Franche-Comté, 16 Route de Gray, 25030 Besançon, France.
Micromachines (Basel). 2024 Oct 12;15(10):1252. doi: 10.3390/mi15101252.
Electrochemical paper-based analytical devices (ePADs) offer an innovative, low-cost, and environmentally friendly approach for real-time diagnostics. In this study, we developed a functional all-inkjet paper-based electrochemical immunosensor using gold (Au) printed ink to detect salivary cortisol. Covalent binding of the cortisol monoclonal antibody onto the printed Au surface was achieved through electrodeposition of 4-carboxymethylaniline (CMA), with ethanolamine passivation to prevent non-specific binding. The ePAD exhibited a linear response within the physiological cortisol range (5-20 ng/mL), with sensitivities of 25, 23, and 19 Ω·ng/mL and R values of 0.995, 0.979, and 0.99, respectively. Additionally, interference studies against tumor necrosis factor-α (TNF-α) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) yielded excellent results. This novel ePAD, fabricated using inkjet printing technology on paper, simplifies the process, reduces environmental impact, and lowers fabrication costs.
基于纸的电化学分析装置（ePADs）为实时诊断提供了一种创新、低成本且环保的方法。在本研究中，我们使用金（Au）印刷油墨开发了一种功能性全喷墨纸基电化学免疫传感器，用于检测唾液皮质醇。通过电沉积4-羧甲基苯胺（CMA）实现皮质醇单克隆抗体与印刷Au表面的共价结合，并使用乙醇胺钝化以防止非特异性结合。该ePAD在生理皮质醇范围内（5-20 ng/mL）表现出线性响应，灵敏度分别为25、23和19 Ω·ng/mL，R值分别为0.995、0.979和0.99。此外，针对肿瘤坏死因子-α（TNF-α）和N端前B型利钠肽（NT-proBNP）的干扰研究取得了优异结果。这种使用喷墨印刷技术在纸上制造的新型ePAD简化了流程，减少了环境影响并降低了制造成本。
